8 Matching Annotations
  1. Feb 2020
    1. Vmax@SGIDNA.com

      Is the genotype of Vmax proprietary? I could not locate any references in the SGI website as well

    1. Vibrio natriegens strain containing a major extracellular nuclease knockout and insertion of an IPTG-inducible T7 RNA polymerase cassette for expression of genes under a T7 promoter.
  2. Jan 2020
    1. To prepare V. natriegens cells for −80 °C storage, an overnight culture of V. natriegens was washed in fresh medium before storage in glycerol
    1. we successfully demonstrated challenging clonings such as Gibson Assembly with 5 and Aquacloning with 3 fragments as well as 8 part golden-gate reactions.
    1. Four inducible promoters were found to function in V. natriegens

      Lac, Pbad (arabinose), lambda promoter

    1. Vmax has a low-level natural resistance to kanamycin but is more susceptible to other antibiotics. When selecting on Kan plates, Vmax with KanR will simply grow faster than Vmax without KanR. Larger colonies=KanR. Other antibiotics select against Vmax but must have a dosage change compared to E. coli.