1,271 Matching Annotations
  1. Apr 2016
    1. P. D. Hsu, E. S. Lander, F. Zhang , Development and applications of CRISPR-Cas9 for genome engineering. Cell 157, 1262–1278 (2014).

      In this extensive review, Feng Zhang discusses in detail the original use of CRIPSR/Cas in bacteria, the timeline for its use in research, and how it has been used for genome editing.

    2. F. Zhang, Y. Wen, X. Guo , CRISPR/Cas9 for genome editing: Progress, implications and challenges. Hum. Mol. Genet. 23, R40–R46(2014).

      The Feng Zhang lab has been instrumental in the development of CRISPR/Cas technology for use in genome engineering. In this review, Zhang summarizes how the tool works, how it can be used for genome editing, regulating gene expression, and for gene therapy as well as some of the limitations/weaknesses that are associated with the technology.

    1. M. R. Looney, B. M. Gilliss, M. A. Matthay, Curr. Opin. Hematol. 17, 418–423 (2010).

      This is a review of clinical findings that explain the cellular responses in TRALI. They also talk about how the interaction between neutrophils and platelets is essential to trigger TRALI.

    2. M. Phillipson et al., J. Exp. Med. 203, 2569–2575 (2006).

      This paper is a good read to see time-lapse videos of intravital imaging and confocal microscopy. It explains how neutrophils require distinct mechanisms to first bind and then crawl out of the vasculature.

  2. Mar 2016
    1. G. W. Kling, G. W. Kipphut, M. M. Miller, J. W. O’Brien, Integration of lakes and streams in a landscape perspective: The importance of material processing on spatial patterns and temporal coherence. Freshw. Biol. 43, 477–497 (2000).

      This study measured how chemical and biological properties of the water in Toolik Lake and its surrounding streams change over time.

      This paper is cited in the supplemental information because the authors used the same analysis methods in the two papers for measuring water chemistry parameters.

    2. Y. Zhang, W. Chen, D. W. Riseborough, Temporal and spatial changes of permafrost in Canada since the end of the Little Ice Age. J. Geophys. Res. 111, D22103 (2006).

      This study used a model to measure the amount of permafrost decrease that occurred between 1850 and 2002 and found that there was enough change to potentially impact landscape, hydrology, and ecosystems.

    3. G. W. Kling, G. W. Kipphut, M. C. Miller, Arctic lakes and streams as gas conduits to the atmosphere: Implications for tundra carbon budgets. Science 251, 298–301 (1991).

      This study showed that carbon from Arctic soils can be released as carbon dioxide from Arctic surface waters.

    4. J. E. Vonk, Ö. Gustafsson, Permafrost-carbon complexities. Nat. Geosci. 6, 675–676 (2013).

      This letter to the editor makes an argument that transport of permafrost carbon into surface waters and beyond is important to consider when modeling the carbon cycle in the Arctic.

    5. D. McGuire, L. G. Anderson, T. R. Christensen, S. Dallimore, L. Guo, D. J. Hayes, M. Heimann, T. D. Lorenson, R. W. Macdonald, N. Roulet, Sensitivity of the carbon cycle in the Arctic to climate change. Ecol. Monogr. 79, 523–555 (2009).

      This review examines the impacts of climate change on the carbon cycle in the Arctic.

    6. C. Tarnocai, J. G. Canadell, E. A. G. Schuur, P. Kuhry, G. Mazhitova, S. Zimov, Soil organic carbon pools in the northern circumpolar permafrost region. Global Biogeochem. Cycles 23, GB2023 (2009).

      This study reported a higher and more reliable estimate of the total amount of carbon stored in Arctic permafrost relative to previous studies. They included deeper soils and additional pools of carbon.

    7. E. A. G. Schuur, J. G. Vogel, K. G. Crummer, H. Lee, J. O. Sickman, T. E. Osterkamp, The effect of permafrost thaw on old carbon release and net carbon exchange from tundra. Nature 459, 556–559 (2009).

      This study found that on a decadal time scale, melting permafrost is a large source of carbon to the atmosphere, even when increases in carbon dioxide use by new plant life is taken into consideration.

    8. R. M. Cory, B. C. Crump, J. A. Dobkowski, G. W. Kling, Surface exposure to sunlight stimulates CO2 release from permafrost soil carbon in the Arctic. Proc. Natl. Acad. Sci. U.S.A. 110, 3429–3434 (2013).

      This study showed that dissolved organic carbon (DOC) that is exposed to sunlight is more susceptible to being broken down by microbial respiration than DOC that is kept in the dark (i.e., photostimulated bacterial respiration is important).

      The authors cite this paper heavily in their supplemental information. Many of the same methods were used in the two papers, including those for field sampling, light absorption measurements, and measurements of photostimulated bacterial respiration.

    9. E. A. G. Schuur, J. Bockheim, J. G. Canadell, E. Euskirchen, C. B. Field, S. V. Goryachkin, S. Hagemann, P. Kuhry, P. M. Lafleur, H. Lee, G. Mazhitova, F. E. Nelson, A. Rinke, V. E. Romanovsky, N. Shiklomanov, C. Tarnocai, S. Venevsky, J. G. Vogel, S. A. Zimov, Vulnerability of permafrost carbon to climate change: Implications for the global carbon cycle. Bioscience 58, 701–714 (2008).

      This review describes the pathways for permafrost carbon to enter the atmosphere and the changes in Arctic ecosystems that could be caused by permafrost thawing. They conclude that the overall impact of thawing permafrost will be to increase atmospheric carbon and global climate change.

    10. H. MacDougall, C. A. Avis, A. J. Weaver, Significant contribution to climate warming from the permafrost carbon feedback. Nat. Geosci. 5, 719–721 (2012).

      This paper uses a model to measure the feedback between global warming and permafrost melting (i.e., how much additional warming will occur because warming is causing additional carbon dioxide and methane to enter the atmosphere).

    11. V. Vähätalo, K. Salonen, U. Münster, M. Järvinen, R. G. Wetzel, Photochemical transformation of allochthonous organic matter provides bioavailable nutrients in a humic lake. Arch. Hydrobiol. 156, 287–314 (2003).

      This study found that sunlight converted dissolved organic carbon, nitrogen, and phosphorous into forms that are more easily consumed by bacteria and plankton in a lake in Norway. The bacteria and plankton were able to be more productive when grown in sunlight-exposed waters.

    12. R. M. Cory, D. M. McKnight, Y.-P. Chin, P. Miller, C. L. Jaros, Chemical characteristics of fulvic acids from Arctic surface waters: Microbial contributions and photochemical transformations. J. Geophys. Res. 112, G04S51 (2007).

      This paper shows that both sunlight and microbial transformation of dissolved organic carbon (DOC) is important to consider when examining the structure of DOC remaining in surface water. Additionally, it is important to consider how long the DOC has been present in the surface water.

    13. Koehler, T. Landelius, G. A. Weyhenmeyer, N. Machida, L. J. Tranvik, Sunlight-induced carbon dioxide emissions from inland waters. Global Biogeochem. Cycles (2014).

      This study is very similar to the one you just read. These authors studied sunlight-driven dissolved organic carbon degradation in Swedish lakes and found that 12% of the carbon dioxide released by the lakes was from photodegradation.

    14. R. M. Cory, K. McNeill, J. P. Cotner, A. Amado, J. M. Purcell, A. G. Marshall, Singlet oxygen in the coupled photochemical and biochemical oxidation of dissolved organic matter. Environ. Sci. Technol. 44, 3683–3689 (2010).

      This study determined the effect of a specific type of sunlight-driven degradation of dissolved organic carbon (DOC) on the availability of DOC to be eaten by bacteria.

      The authors cite this paper in the supplemental information because they used the same methods for measuring oxygen consumption by bacteria in water samples.

    1. S. E. Taylor, J. D. Brown , Illusion and well-being: A social psychological perspective on mental health. Psychol. Bull. 103, 193–210 (1988).

      In this paper, the authors argue that positive self-biases (overly positive self-evaluations, illusion of personal control, and unrealistic optimism) are characteristic of normal human thought.

      In addition, the authors say that these positive illusions are adaptive and beneficial for mental health, in that they promote more satisfying connections with others, greater productivity, and greater resilience in response to negative feedback.

    2. R. E. Nisbett, T. D. Wilson , Telling more than we can know: Verbal reports on mental processes. Psychol. Rev. 84, 231–259 (1977).

      Here, Nisbett and Wilson review evidence that supports the idea that people generally have relatively little insight into their own psychological/cognitive processes. When it comes to the process of a stimulus causing/influencing a response, people can be unaware of the stimulus itself, unaware of the response, or unaware of how the stimulus influenced the response.

      The authors argue that although people can attempt to report on the mechanisms that underlie their thoughts, decisions, etc., these self-reported explanations are not necessarily accurate, and they rely more on people's lay theories about how cognition should work, as opposed to how it actually does work.

    3. S. P. Wojcik, P. H. Ditto , Motivated happiness: Self-enhancement inflates self-reported subjective well-being. Soc. Psychol. Personal. Sci. 5, 825–834 (2014).

      Here, the authors report on the "happier-than-average effect," which shows that greater self-enhancement (either because of individual differences or experimental manipulation) leads to greater reports of subjective well-being.

    4. J. R. Hibbing, K. B. Smith, J. R. Alford , Differences in negativity bias underlie variations in political ideology. Behav. Brain Sci. 37, 297–307 (2014).

      Hibbing, Smith, and Alford argue that individuals' reactions, both physiological and psychological, to negative events and stimuli underlie a major part of the differences between political liberals and conservatives. Their argument centers around the idea that, compared with liberals, conservatives display greater responses to negativity.

    5. M. H. Kernis , Toward a conceptualization of optimal self-esteem. Psychol. Inq. 14, 1–26 (2003).

      This theoretical paper examines the potential for differences between high self-esteem and "optimal" self-esteem. Kernis makes the argument that high self-esteem can be either fragile (nonoptimal) or secure (optimal), depending on factors such as the defensiveness and stability of that self-esteem.

  3. Dec 2015
    1. K. L. Flanagan, R. van Crevel, N. Curtis, F. Shann, O. Levy, Optimmunize Network,

      Researchers have also noted that there are differences in the gender of the child that can change how protective the Measles vaccine is at protecting against other illnesses.

    2. P. Aaby, T. R. Kollmann, C. S. Benn, Nonspecific effects of neonatal and infant vaccination: Public-health, immunological and conceptual challenges. Nat. Immunol. 15, 895–899 (2014).

      WHO and other researchers have found that the Measles vaccine, and other live vaccines, can have effects of childhood mortality not associated with the vaccine target. This reference is a review of some of the studies done in the past to try and better understand this off target effect.

    3. P. Aaby, A. Bhuiya, L. Nahar, K. Knudsen, A. de Francisco, M. Strong, The survival benefit of measles immunization may not be explained entirely by the prevention of measles disease: A community study from rural Bangladesh. Int. J. Epidemiol. 32, 106–115 (2003).

      Scientists use a sample from Bangladesh to look at data similar to the results of this paper. They also find that Measles vaccinating reduces childhood mortality not due to Measles, specifically from diarrhea and oedema. However, the data used in their analysis is only one sample of a population and is confounded by some study control issues.

  4. Nov 2015
    1. B. Rasch, C. Büchel, S. Gais, J. Born , Odor cues during slow-wave sleep prompt declarative memory consolidation. Science 315, 1426–1429 (2007).

      In this study participants were asked to memorize stimuli and were either presented with a smell during the learning process or not. Then during sleep, participants were either re-exposed to the same smell as during learning, a different new smell, or no smell. Participants that were re-exposed to the same smell as during learning had better memory for the items the following morning than either the participants exposed to a new smell or to no smell during sleep.

    2. J. D. Rudoy, J. L. Voss, C. E. Westerberg, K. A. Paller , Strengthening individual memories by reactivating them during sleep. Science 326, 1079 (2009).

      In this study participants learned where to place pictures on a grid on the computer screen. While they learned the locations, each picture was also associated with a sound (e.g. a picture of a bell was also associated with a bell sound). Then, while participants took a nap, half of the sounds were replayed while they slept. After the nap, participants had a better memory for the picture locations of the sounds that were played during sleep than the sounds that were not played during sleep.

    3. M. A. Wilson, B. L. McNaughton , Reactivation of hippocampal ensemble memories during sleep. Science 265, 676–679 (1994).

      In this a seminal study in the area of sleep and memory. Rats have brain cells called 'place cells' that activate when they are in specific locations in their environment. In a series of experiments these researchers demonstrated that place cells that fired in a specific order as the Rat moved around its environment activated in the same order during sleep.

      This was the first evidence that memories may be replayed during sleep after learning.

    4. M. Weisbuch, K. Pauker, N. Ambady , The subtle transmission of race bias via televised nonverbal behavior. Science 326, 1711–1714 (2009).

      In this study researchers extracted short clips from 11 popular television shows, muted the clip so no sound was produced, erased the character that was being interacted with, and asked participants to rate how the other characters were interacting with the 'invisible' character. Results showed that invisible White characters were interacted with more positively than invisible Black characters.

      These same clips were shown to another group of participants and the results showed that exposure to more racially biased nonverbal clips resulted in more implicit bias.

    5. Olsson, J. P. Ebert, M. R. Banaji, E. A. Phelps , The role of social groups in the persistence of learned fear. Science 309, 785–787 (2005).

      In this study researchers presented participants with two Black faces and two White faces. One of the Black faces and one of the White faces was paired wit a mild electric shock (painful but not hurtful) such that each time the participants saw one of the two shock-associated faces they received a shock.

      The researchers then continued to present the faces but stopped giving the shock and measured the fear response.

      The results showed that participants established a fear response to both Black and White shock-associated faces but the fear of the Black shock-associated face persisted while participants stopped fearing the White shock-associated face sooner. When this study was conducted with Black and White participants, for White participants the Black face continued to show a fear response, for Black participants the White face continued to show a fear response.

    6. Gawronski, R. Deutsch, S. Mbirkou, B. Seibt, F. Strack , When “Just Say No” is not enough: Affirmation versus negation training and the reduction of automatic stereotype activation. J. Exp. Soc. Psychol. 44, 370–377 (2008).

      This study compares two types of counterbias training. In one training. In the first type, participants were supposed to respond "YES" when they saw a counter-stereotype pair (e.g. male and weak or female and strong). In the other type of training participants were supposed to respond "NO" when they saw a stereotypical pair (e.g. male and strong or female and weak).

      The results showed that counter-stereotype training (saying yes to counter-stereotypes) DECREASED implicit bias while stereotype negation training (saying no to stereotypical pairs) INCREASED implicit bias.

    7. S. Crandall, A. Eshleman, L. O’Brien , Social norms and the expression and suppression of prejudice: The struggle for internalization. J. Pers. Soc. Psychol. 82, 359–378 (2002).

      In this study, participants rated how appropriate it would be to express prejudice to over 100 different groups (e.g. Black Americans and White Southerners but also liars, child molesters, drunk drivers, and doctors). Then a separate group of participants were asked to rate their personal feelings toward each of the 100 groups.

      The researchers found that willingness to express personal prejudice against a group was strongly related with how acceptable it was to express prejudice against that group.

    8. A. Nosek, F. L. Smyth, N. Sriram, N. M. Lindner, T. Devos, A. Ayala, Y. Bar-Anan, R. Bergh, H. Cai, K. Gonsalkorale, S. Kesebir, N. Maliszewski, F. Neto, E. Olli, J. Park, K. Schnabel, K. Shiomura, B. T. Tulbure, R. W. Wiers, M. Somogyi, N. Akrami, B. Ekehammar, M. Vianello, M. R. Banaji, A. G. Greenwald , National differences in gender-science stereotypes predict national sex differences in science and math achievement. Proc. Natl. Acad. Sci. U.S.A. 106, 10593–10597 (2009).

      In this study more than 500,000 participants completed the gender and academics Implicit Association Test (a test that measures your unconscious associations between different genders (males and females) and different academic areas (liberal arts and sciences).

      The researchers found that countries where participants had higher

      Nations with higher implicit bias scores (associating males with science and females with liberal arts) had larger differences between males' scores and females' scores on an international standardized 8th grade science test.

    9. D. A. Stanley, P. Sokol-Hessner, M. R. Banaji, E. A. Phelps , Implicit race attitudes predict trustworthiness judgments and economic trust decisions. Proc. Natl. Acad. Sci. U.S.A. 108, 7710–7715 (2011).

      In the trust game, participants were presented with a picture of a face and they had to decide whether to offer the person $10 or not. If the participant gave the person $10, the person received $40 and could either give the participant half (so they each received $20) or could keep it all for themselves. Participants saw 300 faces (100 White, 100 Black, and 100 other race) and made the decision to give $10 or not for each face.

      Researchers found that the amount of implicit bias participants had was correlated with how much more likely they were to offer a White person money than a Black person. This was not related to participants' explicit report of racist attitudes.

    10. A. Moss-Racusin, J. F. Dovidio, V. L. Brescoll, M. J. Graham, J. Handelsman , Science faculty’s subtle gender biases favor male students. Proc. Natl. Acad. Sci. U.S.A. 109, 16474–16479 (2012).

      Over 100 science faculty in research universities across the US were asked to determine a starting salary for and rate the application materials of potential research assistants. There were two versions of each application that were identical except that one had a female name and one had a male name. Each faculty member received one of the two versions for each application.

      Faculty members rated the male applications higher and suggested a higher starting salary than the identical female applications.

    11. J. Correll, B. Park, C. M. Judd, B. Wittenbrink, M. S. Sadler, T. Keesee , Across the thin blue line: Police officers and racial bias in the decision to shoot. J. Pers. Soc. Psychol. 92, 1006–1023 (2007).

      In a first-person-shooter videogame participants were instructed to shoot individuals that held a gun and to refrain from shooting individuals that were holding non-gun objects such as wallets or soda cans.

      This finding remained even when experimenters increased the number of White individuals holding guns and decreased the number of Black individuals holding guns.

  5. Oct 2015
    1. Suspendisse auctor pharetra a et hac mauris nisi magna risus taciti a a interdum turpis gravida eu velit magna aliquet a id dignissim. Mi et parturient venenatis quam nullam suspendisse odio mi hac accumsan arcu adipiscing nullam curae habitasse vestibulum

      Ref test 2

  6. Aug 2015
    1. quality-adjusted frontier analysis are available inEckermann and Coelli (2013).

      *Interesting reference for reviewing!

    2. Details of the estimationprocess can be found in operations research textbooks (e.g., Banker, Charnes,and Cooper 1984; Ramanathran and Ramanathan 2003)

      *Interesting reference for reviewing!



    1. H. Wang, M. A. Winnik, I. Manners, Synthesis and self-assembly of poly(ferrocenyldimethylsilane- b -2-vinylpyridine) diblock copolymers. Macromolecules 40, 3784 (2007).

      In this paper, the authors developed a new class of diblock copolymers that have a metal-containing hydrophobic block (PFS) and an organic hydrophilic block (P2VP): PFS = poly(ferrocenyldimethylsilane) and P2VP = poly(2-vinylpyridine). The authors of this publication discovered the ability to obtain spherical and cylindrical morphologies simply by using different alcohols. Having established the ability to obtain cylindrical micelles using the PFS-b-P2VP block copolymer system in isopropyl alcohol, the authors modified their approach in the current study to obtain supermicelles.

    2. P. A. Rupar, G. Cambridge, M. A. Winnik, I. Manners, Reversible cross-linking of polyisoprene coronas in micelles, block comicelles, and hierarchical micelle architectures using Pt(0)–olefin coordination. J. Am. Chem. Soc. 133, 16947 (2011).

      This paper established that Karstedt's catalysts ability to cross-link the double bonds in polyisoprene in the absences of silicon-containing molecules. Besides acquiring a variety of morphologies, the authors also investigated their ability to use Karstedt's catalyst to synthesize reversible polymer gel networks.

    3. X. S. Wanget al., Shell-cross-linked cylindrical polyisoprene- b -polyferrocenylsilane (PI- b -PFS) block copolymer micelles: One-dimensional (1D) organometallic nanocylinders. J. Am. Chem. Soc. 129, 5630 (2007).

      This reference investigates the development of 1D nano-structures through the use of cross-linked cylindrical micelles. This paper highlights possible applications for these 1D nanomaterials such as microfluidics.

    4. R. K. O’Reilly, C. J. Hawker, K. L. Wooley, Cross-linked block copolymer micelles: functional nanostructures of great potential and versatility. Chem. Soc. Rev. 35, 1068 (2006).

      This review paper describes the uses and progress made in the field of cross-linked micelles. Concepts covered include stabilization as well chemical modification and functionalization.

    5. W. Zhanget al., Supramolecular linear heterojunction composed of graphite-like semiconducting nanotubular segments. Science 334, 340 (2011).

      References: This paper describes the synthesis of semiconducting nanotubes through a process similar to CDSA by connecting dissimilar junctions, referred to as heterojuntions, to study the behaviors of photocarriers.

    6. Z.-X. Du, J.-T. Xu, Z.-Q. Fan, Micellar morphologies of poly(ε-caprolactone)- b -poly(ethylene oxide) block copolymers in water with a crystalline core. Macromolecules 40, 7633 (2007).

      This paper describes the use of a biodegradable polymer in order to obtain a variety of micelle morphologies. A concept referred to as tethering density is used in this paper to explain unexpected morphologies.

    7. Schmelz, M. Karg, T. Hellweg, H. Schmalz, General pathway toward crystalline-core micelles with tunable morphology and corona segregation. ACS Nano 5, 9523 (2011).

      This paper uses triblock copolymers to synthesize cylindrical and spherical micelles. By carefully controlling crystallization, the authors were able to control the micellar morphology in a highly selective fashion.

    8. T. Gädt, N. S. Ieong, G. Cambridge, M. A. Winnik, I. Manners, Complex and hierarchical micelle architectures from diblock copolymers using living, crystallization-driven polymerizations. Nat. Mater. 8, 144 (2009).

      This paper utilizes CDSA to synthesize noncylindrical block co-micelles. The authors utilized plateletlike micelle and cylindrical micelles in order to form scarflike architectures using platelet-cylindrical and cylindrical-cylindrical connections.

    9. X. S. Wanget al., Cylindrical block copolymer micelles and co-micelles of controlled length and architecture. Science 317, 644 (2007)

      This paper describes the discovery of CDSA. The authors draw a comparison to living polymerization and explain the phenomenon of epitaxial crystallization-induced co-micellization

    10. Y. Xia, B. D. Olsen, J. A. Kornfield, R. H. Grubbs, Efficient synthesis of narrowly dispersed brush copolymers and study of their assemblies: The importance of side chain arrangement. J. Am. Chem. Soc. 131, 18525 (2009).

      This reference describes the synthesis of brush block and random copolymers. The polymers are referred to as a "brush" because pendant groups are dangling off the main chain. The brush polymers synthesized were amphiphilic and demonstrated self-assembly.

    11. Walther, M. Drechsler, A. H. E. Müller, Structures of amphiphilic Janus discs in aqueous media. Soft Matter 5, 385 (2009).

      This paper describes the synthesis of amphiphilic Janus discs using a block terpolymer (three distinct blocks comprised of three distinct monomers). Two different size discs were made where, depending on size, the manner in which the hydrophobic side is "protected" from water can vary. The smaller discs are stabilized by the long hydrophilic polymer chains, protruding out of one side and shielding the hydrophobic side against water. The larger discs undergo aggregation as well as bending to again shield the hydrophobic side from the water by flipping over one part of the structure.

    12. J. Dupont, G. Liu, ABC triblock copolymer hamburger-like micelles, segmented cylinders, and Janus particles. Soft Matter 6, 3654 (2010).

      This reference is an example where triblock copolymers were photo-crosslinked to create Janus particles which were classified as "hamburger-like" micelles.

    13. Walther, A. H. E. Müller, Janus particles. Soft Matter 4, 663 (2008)

      This review paper discusses a class of noncentrosymmetric nanoparticles referred to as the Janus particle. These particles are rather challenging to synthesize because two different chemistries are present on the surface of the particle.

    14. H. Cui, Z. Chen, S. Zhong, K. L. Wooley, D. J. Pochan, Block copolymer assembly via kinetic control. Science 317, 647 (2007).

      This paper utilized charged polymers as well as metal cations and a variety of solvents to kinetically trap unique micelle morphologies. The self-assembly systems were forced down a specific pathway in order to form morphologies that would not have typically occurred without assistance.

    15. L. Zhang, A. Eisenberg, Multiple morphologies of “crew-cut” aggregates of polystyrene-b-poly(acrylic acid) block copolymers. Science 268, 1728 (1995).

      This paper was one of the first papers to establish the ability to form micelles of different morphologies (beyond just spherical) for systems using amphiphilic block copolymers

  7. Jul 2015
    1. J. F. Soderholm, S. L. Bird, P. Kalab, Y. Sampathkumar, K. Hasegawa, M. Uehara-Bingen, K. Weis, R. Heald, Importazole, a small molecule inhibitor of the transport receptor importin-β. ACS Chem. Biol. 6, 700–708 (2011).

      Importazole is a small molecule inhibitor of the transport receptor importin-β. This inhibitor was identified by a screening assay developed by the authors of this paper.

    2. A. J. Firestone, J. S. Weinger, M. Maldonado, K. Barlan, L. D. Langston, M. O’Donnell, V. I. Gelfand, T. M. Kapoor, J. K. Chen, Small-molecule inhibitors of the AAA+ ATPase motor cytoplasmic dynein. Nature 484, 125–129 (2012). doi:10.1038/nature10936 pmid:22425997

      This work reported the discovery of ciliobrevins, the first specific small-molecule antagonists of cytoplasmic dynein.

    3. K. V. Butler, J. Kalin, C. Brochier, G. Vistoli, B. Langley, A. P. Kozikowski, Rational design and simple chemistry yield a superior, neuroprotective HDAC6 inhibitor, tubastatin A. J. Am. Chem. Soc. 132, 10842–10846 (2010). doi:10.1021/ja102758v pmid:20614936

      This paper reported the development of Tubastatin A, a potent and selective HDAC6 inhibitor.

    4. H. Ouyang, Y. O. Ali, M. Ravichandran, A. Dong, W. Qiu, F. MacKenzie, S. Dhe-Paganon, C. H. Arrowsmith, R. G. Zhai, Protein aggregates are recruited to aggresome by histone deacetylase 6 via unanchored ubiquitin C termini. J. Biol. Chem. 287, 2317–2327 (2012).

      This work suggested a novel ubiquitin-mediated signaling pathway, where the exposure of ubiquitin C termini within protein aggregates enables HDAC6 recognition and transport to the aggresome. The authors found that the ubiquitin-binding domain (ZnF-UBP) of HDAC6, instead of recognizing protein aggregates by binding directly to polyubiquitinated proteins, binds exclusively to the unanchored C-terminal diglycine motif of ubiquitin.

    5. Y. Zhang, B. Gilquin, S. Khochbin, P. Matthias, Two catalytic domains are required for protein deacetylation. J. Biol. Chem. 281, 2401–2404 (2006).

      In this report, the authors showed that both HDAC domains are required for the intact deacetylase activity of HDAC-6.

    6. Y. Zhang, S. Kwon, T. Yamaguchi, F. Cubizolles, S. Rousseaux, M. Kneissel, C. Cao, N. Li, H. L. Cheng, K. Chua, D. Lombard, A. Mizeracki, G. Matthias, F. W. Alt, S. Khochbin, P. Matthias, Mice lacking histone deacetylase 6 have hyperacetylated tubulin but are viable and develop normally. Mol. Cell. Biol. 28, 1688–1701 (2008). doi:10.1128/MCB.01154-06 pmid:18180281

      In this study, the author generated HDAC6 knock out mice and investigated the in vivo functions of HDAC6 and the relevance of tubulin acetylation/deacetylation. they observed that HDAC6-deficient mice are viable and fertile and show hyperacetylated tubulin in most tissues.They concluded that mice survive well without HDAC6 and that tubulin hyperacetylation is not detrimental to normal mammalian development.

    7. I. Kemler, G. Whittaker, A. Helenius, Nuclear import of microinjected influenza virus ribonucleoproteins. Virology 202, 1028–1033 (1994).

This work showed that when influenza virus ribonucleoproteins (vRNPs), devoid of M1, were introduced into the cytoplasm of cells by microinjection, they were found to be imported into the nucleus, and the RNA was transcribed. Their uptake into the nucleus was ATP-dependent, inhibited by antibodies to the nuclear pore complex, unaffected by the prior acidification of the vRNPs, and not inhibited by an anti-virurs, called amantadine. These experiments demonstrated that for productive infection, all the early stages of the viral entry pathway can be bypassed.

    8. anerjee, Y. Yamauchi, A. Helenius, P. Horvath, High-content analysis of sequential events during the early phase of influenza A virus infection. PLOS ONE 8, e68450 (2013).

      This study provides a powerful high-throughput platform to understand the host cell processes. The authors developed quantitative, imaging-based assays to dissect seven consecutive steps in the early phases of IAV infection in tissue culture cells.

    9. K. S. Matlin, H. Reggio, A. Helenius, K. Simons, Infectious entry pathway of influenza virus in a canine kidney cell line. J. Cell Biol. 91, 601–613 (1981).

      This work investigated the cell fusion process of representatives of 3 families of enveloped viruses. it was discovered that hemagglutinin plays a role for the influenza in the low-pH-dependent membrane fusion activity. Low-pH-induced fusion is a widespread property of enveloped animal viruses and that it may play a role in the infective process.

    10. L. H. Pinto, L. J. Holsinger, R. A. Lamb, Influenza virus M2 protein has ion channel activity. Cell 69, 517–528 (1992).

      The authors of this paper identified the ion channel activity of M2.

    11. J. White, K. Matlin, A. Helenius, Cell fusion by Semliki Forest, influenza, and vesicular stomatitis viruses. J. Cell Biol. 89, 674–679 (1981).

      This work investigated the cell fusion process of representatives of 3 families of enveloped viruses. it was discovered that hemagglutinin plays a role for the influenza in the low-pH-dependent membrane fusion activity. Low-pH-induced fusion is a widespread property of enveloped animal viruses and that it may play a role in the infective process.

    12. K. Martin, A. Helenius, Nuclear transport of influenza virus ribonucleoproteins: The viral matrix protein (M1) promotes export and inhibits import. Cell 67, 117–130 (1991). doi:10.1016/0092-8674(91)90576-K pmid:1913813

      This work described the nuclear transport of influenza virus ribonucleoproteins (vRNPs). Viral matrix protein (M1) associates with newly assembled vRNPs in the nucleus and escorts them to the cytoplasm through the nuclear pores. In contrast, during entry of the virus into a new host cell, M1 protein dissociates from the RNPs, allowing them to enter the nucleus.

    1. G. Larson, J. Burger,Trends Genet.29, 197–205 (2013).

      This study is about animal domestication. It explains that the dog is the only animal domesticated before the advent of agriculture. It also discuss the limits of mtDNA analysis.

  8. Oct 2014
    1. The notion behind it was that one could decompose, e.g., Applicative into an instance of the Pointed typeclass and an instance of the Apply typeclass (giving apply :: f (a -> b) -> f a -> f b) and an instance of Pointed, such that the two interact properly.

      There's more on Applicative (and Functor) here, in case you're unfamiliar with it.

  9. Jan 2014
    1. I blogged a while back about how “references” are often described as “addresses” when describing the semantics of the C# memory model. Though that’s arguably correct, it’s also arguably an implementation detail rather than an important eternal truth. Another memory-model implementation detail I often see presented as a fact is “value types are allocated on the stack”. I often see it because of course, that’s what our documentation says.