975 Matching Annotations
  1. Dec 2023
  2. www.biorxiv.org www.biorxiv.org
    A HUMANIZED FCRN TRANSGENIC MOUSE FOR PRECLINICAL PHARMACOKINETICS STUDIES
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    biorxiv.org/content/10.1101/2022.10.24.513622v1
  3. www.jneurosci.org www.jneurosci.org
    T-Type Ca2+ Channels Boost Neurotransmission in Mammalian Cone Photoreceptors
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    jneurosci.org/content/42/33/6325
  4. Oct 2023
  5. www.ncbi.nlm.nih.gov www.ncbi.nlm.nih.gov
    Inhibition of WNK3 kinase signaling reduces brain damage and accelerates neurological recovery after stroke
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    ncbi.nlm.nih.gov/pmc/articles/PMC4643659/
  6. www.ncbi.nlm.nih.gov www.ncbi.nlm.nih.gov
    Deletion of the WNK3-SPAK kinase complex in mice improves radiographic and clinical outcomes in malignant cerebral edema after ischemic stroke
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    ncbi.nlm.nih.gov/pmc/articles/PMC5381450/
  7. Jun 2023
  8. pmc.ncbi.nlm.nih.gov pmc.ncbi.nlm.nih.gov
    Restoration of the gut barrier integrity and restructuring of the gut microbiome in aging by Angiotensin-(1-7)
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    pmc.ncbi.nlm.nih.gov/articles/PMC10881191/
  9. May 2023
  10. www.mdpi.com www.mdpi.com
    [124I]IBETA: A New Aβ Plaque Positron Emission Tomography Imaging Agent for Alzheimer’s Disease
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    mdpi.com/1420-3049/27/14/4552/htm
  11. pubs.acs.org pubs.acs.org
    Novologue Therapy Requires Heat Shock Protein 70 and Thioredoxin-Interacting Protein to Improve Mitochondrial Bioenergetics and Decrease Mitophagy in Diabetic Sensory Neurons
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    pubs.acs.org/doi/10.1021/acschemneuro.1c00340
  12. ejnmmires.springeropen.com ejnmmires.springeropen.com
    [18F]ROStrace detects oxidative stress in vivo and predicts progression of Alzheimer’s disease pathology in APP/PS1 mice
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    ejnmmires.springeropen.com/articles/10.1186/s13550-022-00914-x
  13. pmc.ncbi.nlm.nih.gov pmc.ncbi.nlm.nih.gov
    Differential Responses to Sigma-1 or Sigma-2 Receptor Ablation in Adiposity, Fat Oxidation, and Sexual Dimorphism
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    pmc.ncbi.nlm.nih.gov/articles/PMC9506228/
  14. asbmr.onlinelibrary.wiley.com asbmr.onlinelibrary.wiley.com
    Germline Saturation Mutagenesis Induces Skeletal Phenotypes in Mice
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    asbmr.onlinelibrary.wiley.com/doi/full/10.1002/jbmr.4323
  15. www.embopress.org www.embopress.org
    Aire-dependent transcripts escape Raver2-induced splice-event inclusion in the thymic epithelium
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    embopress.org/doi/full/10.15252/embr.202153576
  16. www.embopress.org www.embopress.org
    PKM2-TMEM33 axis regulates lipid homeostasis in cancer cells by controlling SCAP stability
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    embopress.org/doi/full/10.15252/embj.2021108065
  17. anatomypubs.onlinelibrary.wiley.com anatomypubs.onlinelibrary.wiley.com
    Glypican-6 and Glypican-4 stimulate embryonic stomach growth by regulating Hedgehog and non-canonical Wnt signaling
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    anatomypubs.onlinelibrary.wiley.com/doi/full/10.1002/dvdy.533
  18. bpspubs.onlinelibrary.wiley.com bpspubs.onlinelibrary.wiley.com
    5-HT3Rs Maintain Hippocampal LTP in a CB1R-GABAA-Dependent Manner for Spatial Memory
    1
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    bpspubs.onlinelibrary.wiley.com/doi/full/10.1111/bph.15793
  19. faseb.onlinelibrary.wiley.com faseb.onlinelibrary.wiley.com
    Ablation of PDE4B protects from Pseudomonas aeruginosa-induced acute lung injury in mice by ameliorating the cytostorm and associated hypothermia
    1
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    faseb.onlinelibrary.wiley.com/doi/full/10.1096/fj.202100495R
  20. onlinelibrary.wiley.com onlinelibrary.wiley.com
    Oligodendrogenesis is a key process for cognitive performance improvement induced by voluntary physical activity
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    onlinelibrary.wiley.com/doi/full/10.1002/glia.24155
  21. faseb.onlinelibrary.wiley.com faseb.onlinelibrary.wiley.com
    Genetic deletion of Cyp4f18 disrupts the omega-3 epoxidation pathway and results in psoriasis-like dermatitis
    1
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    faseb.onlinelibrary.wiley.com/doi/full/10.1096/fj.202200982R
  22. Apr 2023
  23. onlinelibrary.wiley.com onlinelibrary.wiley.com
    Erythroferrone contributes to iron mobilization for embryo erythropoiesis in iron-deficient mouse pregnancies
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    onlinelibrary.wiley.com/doi/full/10.1002/ajh.26680
  24. www.biorxiv.org www.biorxiv.org
    Multiple dynamic interactions from basal ganglia direct and indirect pathways mediate action selection
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    biorxiv.org/content/10.1101/2023.03.20.533567v3
  25. www.ncbi.nlm.nih.gov www.ncbi.nlm.nih.gov
    The Striatal Balancing Act in Drug Addiction: Distinct Roles of Direct and Indirect Pathway Medium Spiny Neurons
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    ncbi.nlm.nih.gov/pmc/articles/PMC3140647/
  26. www.ncbi.nlm.nih.gov www.ncbi.nlm.nih.gov
    Genetic quality assurance and genetic monitoring of laboratory mice and rats: FELASA Working Group Report
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    ncbi.nlm.nih.gov/pmc/articles/PMC7160752/
  27. www.ncbi.nlm.nih.gov www.ncbi.nlm.nih.gov
    The Monarch Initiative in 2019: an integrative data and analytic platform connecting phenotypes to genotypes across species
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    ncbi.nlm.nih.gov/pmc/articles/PMC7056945/
  28. www.ncbi.nlm.nih.gov www.ncbi.nlm.nih.gov
    Modeling Genetic Susceptibility to Disease
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    ncbi.nlm.nih.gov/pmc/articles/PMC7152220/
  29. www.ncbi.nlm.nih.gov www.ncbi.nlm.nih.gov
    Biology and Diseases of Mice
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    ncbi.nlm.nih.gov/pmc/articles/PMC7150197/
  30. www.ncbi.nlm.nih.gov www.ncbi.nlm.nih.gov
    From Mice to Humans
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    ncbi.nlm.nih.gov/pmc/articles/PMC3488608/
  31. www.ncbi.nlm.nih.gov www.ncbi.nlm.nih.gov
    Tet-Transgenic Rodents: a comprehensive, up-to date database
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    ncbi.nlm.nih.gov/pmc/articles/PMC3591533/
  32. www.ncbi.nlm.nih.gov www.ncbi.nlm.nih.gov
    33rd international mammalian genome conference: meeting highlights
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    ncbi.nlm.nih.gov/pmc/articles/PMC7087741/
  33. www.ncbi.nlm.nih.gov www.ncbi.nlm.nih.gov
    27th International Mammalian Genome Conference meeting report
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    ncbi.nlm.nih.gov/pmc/articles/PMC7088217/
  34. www.ncbi.nlm.nih.gov www.ncbi.nlm.nih.gov
    The Mammalian Phenotype Ontology as a unifying standard for experimental and high-throughput phenotyping data
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    ncbi.nlm.nih.gov/pmc/articles/PMC3463787/
  35. www.ncbi.nlm.nih.gov www.ncbi.nlm.nih.gov
    Critical Roles of Dual-Specificity Phosphatases in Neuronal Proteostasis and Neurological Diseases
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    ncbi.nlm.nih.gov/pmc/articles/PMC5618612/
  36. www.ncbi.nlm.nih.gov www.ncbi.nlm.nih.gov
    Methodologies and Challenges for CRISPR/Cas9 Mediated Genome Editing of the Mammalian Brain
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    ncbi.nlm.nih.gov/pmc/articles/PMC8525404/
  37. www.ncbi.nlm.nih.gov www.ncbi.nlm.nih.gov
    The mammalian gene function resource: the international knockout mouse consortium
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    ncbi.nlm.nih.gov/pmc/articles/PMC3463800/
  38. www.ncbi.nlm.nih.gov www.ncbi.nlm.nih.gov
    A resource of vectors and ES cells for targeted deletion of microRNAs in mice
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    ncbi.nlm.nih.gov/pmc/articles/PMC3242032/
  39. www.ncbi.nlm.nih.gov www.ncbi.nlm.nih.gov
    Cryopreservation of mouse resources
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    ncbi.nlm.nih.gov/pmc/articles/PMC7495967/
  40. www.ncbi.nlm.nih.gov www.ncbi.nlm.nih.gov
    The mouse genetics toolkit: revealing function and mechanism
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    ncbi.nlm.nih.gov/pmc/articles/PMC3218837/
  41. www.ncbi.nlm.nih.gov www.ncbi.nlm.nih.gov
    CreZOO—the European virtual repository of Cre and other targeted conditional driver strains
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    ncbi.nlm.nih.gov/pmc/articles/PMC3381224/
  42. www.ncbi.nlm.nih.gov www.ncbi.nlm.nih.gov
    The Mouse Universal Genotyping Array: From Substrains to Subspecies
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    ncbi.nlm.nih.gov/pmc/articles/PMC4751547/
  43. www.ncbi.nlm.nih.gov www.ncbi.nlm.nih.gov
    Saturation mutagenesis defines novel mouse models of severe spine deformity
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    ncbi.nlm.nih.gov/pmc/articles/PMC8246263/
  44. Mar 2023
  45. www.ncbi.nlm.nih.gov www.ncbi.nlm.nih.gov
    Peroxisomes Regulate Cellular Free Fatty Acids to Modulate Mast Cell TLR2, TLR4, and IgE-Mediated Activation
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    ncbi.nlm.nih.gov/pmc/articles/PMC9215104/
  46. www.jneurosci.org www.jneurosci.org
    Mllt11 Regulates Migration and Neurite Outgrowth of Cortical Projection Neurons during Development
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    jneurosci.org/content/42/19/3931
  47. journals.biologists.com journals.biologists.com
    Focal adhesion kinase-mediated signaling controls the onset of pancreatic cell differentiation
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    journals.biologists.com/dev/article/149/17/dev200761/276568/Focal-adhesion-kinase-mediated-signaling-controls
  48. www.biorxiv.org www.biorxiv.org
    Neuroinvasive flavivirus pathogenesis is restricted by host genetic factors in Collaborative Cross mice, independently of Oas1b
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    biorxiv.org/content/10.1101/2022.10.24.513634v1
  49. www.nature.com www.nature.com
    3R measures in facilities for the production of genetically modified rodents
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    nature.com/articles/s41684-022-00978-1
  50. www.science.org www.science.org
    The Candida albicans toxin candidalysin mediates distinct epithelial inflammatory responses through p38 and EGFR-ERK pathways
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    science.org/doi/10.1126/scisignal.abj6915
  51. pmc.ncbi.nlm.nih.gov pmc.ncbi.nlm.nih.gov
    TWIST1 interacts with β/δ-catenins during neural tube development and regulates fate transition in cranial neural crest cells
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    pmc.ncbi.nlm.nih.gov/articles/PMC9440756/
  52. www.ncbi.nlm.nih.gov www.ncbi.nlm.nih.gov
    A requirement for Krüppel Like Factor‐4 in the maintenance of endothelial cell quiescence
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    ncbi.nlm.nih.gov/pmc/articles/PMC9679496/
  53. journals.plos.org journals.plos.org
    Shp2 in uterine stromal cells critically regulates on time embryo implantation and stromal decidualization by multiple pathways during early pregnancy
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    journals.plos.org/plosgenetics/article
  54. www.nature.com www.nature.com
    Distinct roles of KLF4 in mesenchymal cell subtypes during lung fibrogenesis
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    nature.com/articles/s41467-021-27499-8
  55. www.nature.com www.nature.com
    FAM72A antagonizes UNG2 to promote mutagenic repair during antibody maturation
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    nature.com/articles/s41586-021-04144-4
  56. aacrjournals.org aacrjournals.org
    Mice Deficient in the RNA-Binding Protein Zfp871 Are Prone to Early Death and Steatohepatitis in Part through the p53–Mdm2 Axis
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    aacrjournals.org/mcr/article/19/10/1751/665681/Mice-Deficient-in-the-RNA-Binding-Protein-Zfp871
  57. www.biorxiv.org www.biorxiv.org
    Dopaminergic axons track somatic signaling in behaving mice
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    biorxiv.org/content/10.1101/2022.06.20.496872v1
  58. cgp.iiarjournals.org cgp.iiarjournals.org
    Fucoxanthinol Induces Apoptosis in a Pancreatic Intraepithelial Neoplasia Cell Line
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    cgp.iiarjournals.org/content/18/2/133
  59. www.nature.com www.nature.com
    Dual roles of hexokinase 2 in shaping microglial function by gating glycolytic flux and mitochondrial activity
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    nature.com/articles/s42255-022-00707-5
  60. www.biorxiv.org www.biorxiv.org
    Modulation of dLGN firing mode across multiple timescales is predicted by pupil size dynamics
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    biorxiv.org/content/10.1101/2021.04.30.442134v2
  61. www.jci.org www.jci.org
    The ZIP8/SIRT1 axis regulates alveolar progenitor cell renewal in aging and idiopathic pulmonary fibrosis
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    jci.org/articles/view/157338
  62. www.nature.com www.nature.com
    Revealing nanostructures in brain tissue via protein decrowding by iterative expansion microscopy
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    nature.com/articles/s41551-022-00912-3
  63. www.jci.org www.jci.org
    A Piezo1/KLF15/IL-6 axis mediates immobilization-induced muscle atrophy
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    jci.org/articles/view/154611
  64. link.springer.com link.springer.com
    Chemerin regulates normal angiogenesis and hypoxia-driven neovascularization
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    link.springer.com/article/10.1007/s10456-021-09818-1
  65. link.springer.com link.springer.com
    Mouse Genome Informatics (MGI): latest news from MGD and GXD
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    link.springer.com/article/10.1007/s00335-021-09921-0
  66. www.sciencedirect.com www.sciencedirect.com
    Reduced housing density improves statistical power of murine gut microbiota studies
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    sciencedirect.com/science/article/pii/S2211124722005502
  67. www.biorxiv.org www.biorxiv.org
    Modulating D1 rather than D2 receptor-expressing spiny-projection neurons corresponds to optimal antipsychotic effect
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    biorxiv.org/content/10.1101/2021.08.03.454992v1
  68. link.springer.com link.springer.com
    A dominant negative mutation uncovers cooperative control of caudal Wolffian duct development by Sprouty genes
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    link.springer.com/article/10.1007/s00018-022-04546-1
  69. www.ncbi.nlm.nih.gov www.ncbi.nlm.nih.gov
    FoxA-dependent DNA demethylation initiates epigenetic control of cellular stability
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    ncbi.nlm.nih.gov/pmc/articles/PMC8129911/
  70. www.biorxiv.org www.biorxiv.org
    A neural substrate of sex-dependent modulation of motivation by value
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    biorxiv.org/content/10.1101/2022.07.07.499209v1
  71. www.science.org www.science.org
    Sex-specific phenotypic effects and evolutionary history of an ancient polymorphic deletion of the human growth hormone receptor
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    science.org/doi/10.1126/sciadv.abi4476
  72. www.nature.com www.nature.com
    PERK is a critical metabolic hub for immunosuppressive function in macrophages
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    nature.com/articles/s41590-022-01145-x
  73. rupress.org rupress.org
    Loss of direct adrenergic innervation after peripheral nerve injury causes lymph node expansion through IFN-γ
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    rupress.org/jem/article/218/8/e20202377/212257/Loss-of-direct-adrenergic-innervation-after
  74. www.sciencedirect.com www.sciencedirect.com
    MeCP2 controls neural stem cell fate specification through miR-199a-mediated inhibition of BMP-Smad signaling
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    sciencedirect.com/science/article/pii/S2211124721004630
  75. www.biorxiv.org www.biorxiv.org
    Tapping into the aging brain: In vivo microdialysis reveals mirroring pathology between preclinical models and patients with Alzheimer’s disease
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    biorxiv.org/content/10.1101/2021.01.23.427888v1
  76. link.springer.com link.springer.com
    Insight into the Animal Models for Microbiome Studies
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    link.springer.com/chapter/10.1007/978-981-16-3156-6_13
  77. www.sciencedirect.com www.sciencedirect.com
    BMPR1a Is Required for the Optimal TGFβ1-Dependent CD207+ Langerhans Cell Differentiation and Limits Skin Inflammation through CD11c+ Cells
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    sciencedirect.com/science/article/pii/S0022202X22001956
  78. www.biorxiv.org www.biorxiv.org
    Neprilysin-sensitive amyloidogenic Aβ versus IDE-sensitive soluble Aβ: a probable mechanistic cause for sporadic Alzheimer’s disease
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    biorxiv.org/content/10.1101/2021.08.22.457281v1
  79. www.jci.org www.jci.org
    DACH1 protects podocytes from experimental diabetic injury and modulates PTIP-H3K4Me3 activity
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    jci.org/articles/view/141279
  80. www.biorxiv.org www.biorxiv.org
    Single Cell Enhancer Activity Maps Neuronal Lineages in Embryonic Mouse Basal Ganglia
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    biorxiv.org/content/10.1101/2021.01.11.426285v1
  81. www.mdpi.com www.mdpi.com
    Investigation of Anxiety- and Depressive-like Symptoms in 4- and 8-Month-Old Male Triple Transgenic Mouse Models of Alzheimer’s Disease
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    mdpi.com/1422-0067/23/18/10816
  82. www.life-science-alliance.org www.life-science-alliance.org
    Mitochondrial stress-induced GFRAL signaling controls diurnal food intake and anxiety-like behavior
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    life-science-alliance.org/content/5/11/e202201495
  83. www.biorxiv.org www.biorxiv.org
    Mitochondrial stress-induced GDF15-GFRAL axis promotes anxiety-like behavior and CRH-dependent anorexia
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    biorxiv.org/content/10.1101/2021.09.22.461199v1
  84. journals.biologists.com journals.biologists.com
    Promoting validation and cross-phylogenetic integration in model organism research
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    journals.biologists.com/dmm/article/15/9/dmm049600/276675/Promoting-validation-and-cross-phylogenetic
  85. www.biorxiv.org www.biorxiv.org
    Opposing regulation of short-term memory by basal ganglia direct and indirect pathways that are coactive during behavior
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    biorxiv.org/content/10.1101/2021.12.15.472735v1.full.pdf
  86. www.frontiersin.org www.frontiersin.org
    The Therapeutic and Diagnostic Potential of Amyloid β Oligomers Selective Antibodies to Treat Alzheimer’s Disease
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    frontiersin.org/articles/10.3389/fnins.2021.768646/full
  87. pmc.ncbi.nlm.nih.gov pmc.ncbi.nlm.nih.gov
    Retinal Photoreceptor Protection in an AMD-Related Mouse Model by Selective Sigma-1 or Sigma-2 Receptor Modulation
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    pmc.ncbi.nlm.nih.gov/articles/PMC9778362/
  88. link.springer.com link.springer.com
    Genetic quality: a complex issue for experimental study reproducibility
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    link.springer.com/article/10.1007/s11248-022-00314-w
  89. www.nature.com www.nature.com
    Astrocytic interleukin-3 programs microglia and limits Alzheimer’s disease
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    nature.com/articles/s41586-021-03734-6
  90. www.sciencedirect.com www.sciencedirect.com
    Deficiency of the sedoheptulose kinase (Shpk) does not alter the ability of hematopoietic stem cells to rescue cystinosis in the mouse model
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    sciencedirect.com/science/article/pii/S1096719221008246
  91. www.mdpi.com www.mdpi.com
    Consideration of Gut Microbiome in Murine Models of Diseases
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    mdpi.com/2076-2607/9/5/1062
  92. www.nature.com www.nature.com
    Single-cell analysis of skin immune cells reveals an Angptl4-ifi20b axis that regulates monocyte differentiation during wound healing
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    nature.com/articles/s41419-022-04638-7
  93. www.sciencedirect.com www.sciencedirect.com
    A novel DPH5-related diphthamide-deficiency syndrome causing embryonic lethality or profound neurodevelopmental disorder
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    sciencedirect.com/science/article/pii/S1098360022007043
  94. www.biorxiv.org www.biorxiv.org
    Embigin deficiency leads to delayed embryonic lung development and high neonatal mortality
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  159. Apr 2022
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  186. www.ncbi.nlm.nih.gov www.ncbi.nlm.nih.gov
    Netrin-5 is highly expressed in neurogenic regions of the adult brain
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    ncbi.nlm.nih.gov/pmc/articles/PMC4403520/
  187. www.ncbi.nlm.nih.gov www.ncbi.nlm.nih.gov
    Cell-Type-Specific Afferent Innervation of the Nucleus Accumbens Core and Shell
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    ncbi.nlm.nih.gov/pmc/articles/PMC6232828/
  188. www.ncbi.nlm.nih.gov www.ncbi.nlm.nih.gov
    Whole-Brain Monosynaptic Afferent Inputs to Basal Forebrain Cholinergic System
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    ncbi.nlm.nih.gov/pmc/articles/PMC5056182/
  189. www.ncbi.nlm.nih.gov www.ncbi.nlm.nih.gov
    Green-Fluorescent Protein+ Astrocytes Attach to Beta-Amyloid Plaques in an Alzheimer Mouse Model and Are Sensitive for Clasmatodendrosis
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    ncbi.nlm.nih.gov/pmc/articles/PMC4825400/
  190. www.ncbi.nlm.nih.gov www.ncbi.nlm.nih.gov
    Respiratory Immunization With a Whole Cell Inactivated Vaccine Induces Functional Mucosal Immunoglobulins Against Tuberculosis in Mice and Non-human Primates
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    ncbi.nlm.nih.gov/pmc/articles/PMC7315045/
  191. www.ncbi.nlm.nih.gov www.ncbi.nlm.nih.gov
    Interleukin-22 From Type 3 Innate Lymphoid Cells Aggravates Lupus Nephritis by Promoting Macrophage Infiltration in Lupus-Prone Mice
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    ncbi.nlm.nih.gov/pmc/articles/PMC7953152/
  192. www.ncbi.nlm.nih.gov www.ncbi.nlm.nih.gov
    Lymph Flow Induces the Postnatal Formation of Mature and Functional Meningeal Lymphatic Vessels
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    ncbi.nlm.nih.gov/pmc/articles/PMC6970982/
  193. www.ncbi.nlm.nih.gov www.ncbi.nlm.nih.gov
    Ezh2 Regulates Activation-Induced CD8+ T Cell Cycle Progression via Repressing Cdkn2a and Cdkn1c Expression
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    ncbi.nlm.nih.gov/pmc/articles/PMC5879148/
  194. www.ncbi.nlm.nih.gov www.ncbi.nlm.nih.gov
    Cre-Lox Neurogenetics: 20 Years of Versatile Applications in Brain Research and Counting…
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    ncbi.nlm.nih.gov/pmc/articles/PMC4759636/
  195. www.ncbi.nlm.nih.gov www.ncbi.nlm.nih.gov
    Upregulation of Mir342 in Diet-Induced Obesity Mouse and the Hypothalamic Appetite Control
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    ncbi.nlm.nih.gov/pmc/articles/PMC8437242/
  196. www.ncbi.nlm.nih.gov www.ncbi.nlm.nih.gov
    Mouse Retinal Organoid Growth and Maintenance in Longer-Term Culture
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    ncbi.nlm.nih.gov/pmc/articles/PMC8114082/
  197. www.ncbi.nlm.nih.gov www.ncbi.nlm.nih.gov
    NKAP Regulates Senescence and Cell Death Pathways in Hematopoietic Progenitors
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    ncbi.nlm.nih.gov/pmc/articles/PMC6783958/
  198. www.ncbi.nlm.nih.gov www.ncbi.nlm.nih.gov
    Sexual Dimorphism in the 3xTg-AD Mouse Model and Its Impact on Pre-Clinical Research
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    ncbi.nlm.nih.gov/pmc/articles/PMC8075398/
  199. www.ncbi.nlm.nih.gov www.ncbi.nlm.nih.gov
    Anticoagulants inhibit proteolytic clearance of plasma amyloid beta
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    ncbi.nlm.nih.gov/pmc/articles/PMC5814162/
  200. www.ncbi.nlm.nih.gov www.ncbi.nlm.nih.gov
    The essential role of GATA transcription factors in adult murine prostate
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    ncbi.nlm.nih.gov/pmc/articles/PMC5216986/
  201. www.ncbi.nlm.nih.gov www.ncbi.nlm.nih.gov
    Loss of angiotensin converting enzyme II (ACE2) accelerates the development of liver injury induced by thioacetamide
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    ncbi.nlm.nih.gov/pmc/articles/PMC5814313/