Reviewer #1 (Jerry Workman):

This is a paradigm shifting study which demonstrates targeting of the Isw2 complex by a sequence-specific DNA binding protein Ume6. Previously the Isw2 complex was thought to be a promiscuous nucleosome sliding ATPase that would globally space nucleosomes like Chd1 or Isw1. However, the current study demonstrates the Isw2 primarily targets a single nucleosome adjacent to Ume6 binding sites.