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  1. May 2019
  2. shodhganga.inflibnet.ac.in shodhganga.inflibnet.ac.in
    12_chapter 2.pdf
    2
    1. shivamojha 16 May 2019
      Aftereffluentexposure,thetissuesweredissectedout,weighedandhomogenizedin0.25Msucrose.Thehomogenateswerecentrifugedat10,000rev/minatatemperaturebelow8°C.AcetylcholinesteraseactivityofthesampleswasdeterminedatpH7.0usingafinalhomogenateconcentrationof25mgmf1at10°CwithmMacetylcholineiodideassubstrate and0.001or0.002NsodiumhydroxideastitrantfollowingHestron’smethodasgivenbyMetcalf(1951).ProteindeterminationsforalltheChEanalyseswereconductedonaliquotsofthehomogenatesusingamodificationoftheLowryetal.(1951)method.AchEactivityisexpressedinpmolesofacetylcholinechloridehydrolysedmgtissue'1hr'1
      Method-17-Method-2-detail
    2. shivamojha 16 May 2019
      Acetylcholinesterase(AchE
      Method-17-Method-2
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    shodhganga.inflibnet.ac.in/bitstream/10603/104502/12/12_chapter 2.pdf