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  1. May 2019
  2. shodhganga.inflibnet.ac.in shodhganga.inflibnet.ac.in
    12_chapter 2.pdf
    2
    1. shivamojha 16 May 2019
      Thesupernatant(0.5mlcontaining50mgtissue)wasassayedforSDH.Thereactionwasinitiatedbytheadditionof0.5mlofthesupernatant.Controlsreceived0.5mlsucroseinplaceoftheenzymeextract.Afterincubationfor30minat37°C,thereactionwasstoppedbytheadditionof5mlglacialaceticacidandthederivedformazanwasextractedinto5mloftoluene.Afterkeepingitovernightincold,thecolorwasmeasuredinUV-Spectrophotometerat495mMusingsilicacuvettes.Enzymeactivitieswereexpressedaspmolesofformazanmg'1proteinhr'1
      Method-17-Method-4-detail
    2. shivamojha 16 May 2019
      Thisisanimportantenzymeinvolvedinthecitricacidcycle.Thehomogenatesofcontrolandeffluentexposedtissueswerepreparedin0.25MicecoldsucroseusingPotterElvehjemtypeglasshomogenizerandcentrifugedat3000rpmfor15min
      Method-17-Method-4-detail
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    shodhganga.inflibnet.ac.in/bitstream/10603/104502/12/12_chapter 2.pdf