5 Matching Annotations
- May 2019
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shodhganga.inflibnet.ac.in shodhganga.inflibnet.ac.in
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The RIA for alpha hCG was similar to that used to estimate ~hCG. A monoclonal antibody specific to alpha hCG ( Gupta et al. , 1985 was used for the assay. The standard used was total hCG.
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RIA for alpha hCG.
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shodhganga.inflibnet.ac.in shodhganga.inflibnet.ac.in
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ultrapureHNO3andtissuesamplesweredissolvedin70%HNO3;microwavedfor5minat90W,180W,270Wand360W,untiltotaldigestionhadoccurredandthendilutedwithMilli-Qgradewater(Millipore,Acton,Massachusetts,U.S.A)
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Totalsodium,potassiumandcalciumconcentrationsweredeterminedwithatomicabsorptionspectrophotometry.Tothispurpose,plasmasamplesweredilutedwith1%
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Ionconcentrations
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