7 Matching Annotations
  1. Jun 2025
  2. pmc.ncbi.nlm.nih.gov pmc.ncbi.nlm.nih.gov
    A streamlined tandem affinity purification of His-MBP-SpyCas9, without buffer exchange, suitable for in vitro cleavage applications
    2
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    pmc.ncbi.nlm.nih.gov/articles/PMC12143660/
  3. Sep 2024
  4. www.ncbi.nlm.nih.gov www.ncbi.nlm.nih.gov
    CRISPR-Cas9 target-strand nicking provides phage resistance by inhibiting replication
    1
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    ncbi.nlm.nih.gov/pmc/articles/PMC11398490/
  5. Jan 2024
  6. academic.oup.com academic.oup.com
    Systematic in vitro specificity profiling reveals nicking defects in natural and engineered CRISPR–Cas9 variants
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    academic.oup.com/nar/advance-article/doi/10.1093/nar/gkab163/6179346
  7. Dec 2023
  8. www.ncbi.nlm.nih.gov www.ncbi.nlm.nih.gov
    Cas9 is mostly orthogonal to human systems of DNA break sensing and repair
    1
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    ncbi.nlm.nih.gov/pmc/articles/PMC10686484/
  9. Jul 2021
  10. elifesciences.org elifesciences.org
    Structural and functional insights into the bona fide catalytic state of Streptococcus pyogenes Cas9 HNH nuclease domain
    1
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    elifesciences.org/articles/46500
  11. Mar 2021
  12. academic.oup.com academic.oup.com
    Systematic in vitro specificity profiling reveals nicking defects in natural and engineered CRISPR–Cas9 variants
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    academic.oup.com/nar/advance-article/doi/10.1093/nar/gkab163/6179346