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Aug 2026
pmc.ncbi.nlm.nih.gov pmc.ncbi.nlm.nih.gov

Sorbose metabolism promotes fitness and virulence in Escherichia coli

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1. scibot 08 Aug 2026
  
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  addgene_44251
  
  DOI: 10.1128/msphere.00191-26
  
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pmc.ncbi.nlm.nih.gov/articles/PMC13410766/
Jun 2026
pmc.ncbi.nlm.nih.gov pmc.ncbi.nlm.nih.gov

Development and Optimization of CRISPR/Cas9-Assisted Recombineering in Escherichia albertii

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1. scibot 06 Jun 2026
  
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  44251
  
  DOI: 10.17912/micropub.biology.002076
  
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pmc.ncbi.nlm.nih.gov/articles/PMC13187952/
Aug 2025
pmc.ncbi.nlm.nih.gov pmc.ncbi.nlm.nih.gov

CRISPRi screening reveals E. coli’s anaerobic-like respiratory adaptations to gentamicin: membrane depolarization by CpxR

1
1. scibot 11 Aug 2025
  
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  44251
  
  DOI: 10.1128/msystems.00353-25
  
  Resource: RRID:Addgene_44251
  
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pmc.ncbi.nlm.nih.gov/articles/PMC12282077/
Jul 2025
pmc.ncbi.nlm.nih.gov pmc.ncbi.nlm.nih.gov

The Role of L-Sorbose Metabolism in Enhancing Fitness and Virulence in Escherichia coli Under Acidic Conditions

1
1. scibot 20 Jul 2025
  
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  Addgene_44251
  
  DOI: 10.2147/IDR.S526664
  
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pmc.ncbi.nlm.nih.gov/articles/PMC12186808/
May 2025
pmc.ncbi.nlm.nih.gov pmc.ncbi.nlm.nih.gov

Rapid identification of key antibiotic resistance genes in E. coli using high-resolution genome-scale CRISPRi screening

1
1. scibot 17 May 2025
  
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  44251
  
  DOI: 10.1016/j.isci.2025.112435
  
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pmc.ncbi.nlm.nih.gov/articles/PMC12063145/
pmc.ncbi.nlm.nih.gov pmc.ncbi.nlm.nih.gov

Use of Whole Cells and Cell‐Free Extracts of Catalase‐Deficient E. coli for Peroxygenase‐Catalyzed Reactions

1
1. scibot 17 May 2025
  
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  plasmid_44251
  
  DOI: 10.1002/bit.28959
  
  Resource: RRID:Addgene_44251
  
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pmc.ncbi.nlm.nih.gov/articles/PMC12067033/
Apr 2025
pmc.ncbi.nlm.nih.gov pmc.ncbi.nlm.nih.gov

Assessing the feasibility of CRISPRa approaches to enhance protein-based biomaterial expression in bacterial systems for more efficient production

1
1. scibot 18 Apr 2025
  
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  RRID:Addgene_44251
  
  DOI: 10.1016/j.mtbio.2025.101720
  
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pmc.ncbi.nlm.nih.gov/articles/PMC11997408/
Mar 2025
pmc.ncbi.nlm.nih.gov pmc.ncbi.nlm.nih.gov

Metabolic mutations reduce antibiotic susceptibility of E. coli by pathway-specific bottlenecks

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1. scibot 11 Mar 2025
  
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  Addgene_44251
  
  DOI: 10.1038/s44320-024-00084-z
  
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pmc.ncbi.nlm.nih.gov/articles/PMC11876631/
Sep 2024
www.ncbi.nlm.nih.gov www.ncbi.nlm.nih.gov

Re-engineered guide RNA enables DNA loops and contacts modulating repression in E. coli

1
1. scibot 29 Sep 2024
  
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  44 251
  
  DOI: 10.1093/nar/gkae591
  
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  RRID:Addgene_44251 DOI:10.1093/nar/gkae591
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ncbi.nlm.nih.gov/pmc/articles/PMC11347156/
Jun 2024
www.sciencedirect.com www.sciencedirect.com

A metabolomics pipeline highlights microbial metabolism in bloodstream infections

1
1. scibot 21 Jun 2024
  
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  Addgene_44251
  
  DOI: 10.1016/j.cell.2024.05.035
  
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sciencedirect.com/science/article/pii/S0092867424005798
Apr 2024
www.ncbi.nlm.nih.gov www.ncbi.nlm.nih.gov

CRISPR-COPIES: an in silico platform for discovery of neutral integration sites for CRISPR/Cas-facilitated gene integration

1
1. scibot 23 Apr 2024
  
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  44251
  
  DOI: 10.1093/nar/gkae062
  
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ncbi.nlm.nih.gov/pmc/articles/PMC11014336/
www.ncbi.nlm.nih.gov www.ncbi.nlm.nih.gov

Combining CRISPR/Cas9 and natural excision for the precise and complete removal of mobile genetic elements in bacteria

1
1. scibot 23 Apr 2024
  
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  pgRNA-bacteria
  
  DOI: 10.1128/aem.00095-24
  
  Resource: RRID:Addgene_44251
  
  Curator: @olekpark
  
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ncbi.nlm.nih.gov/pmc/articles/PMC11022536/
Oct 2022
www.sciencedirect.com www.sciencedirect.com

CasPlay provides a gRNA-barcoded CRISPR-based display platform for antibody repertoire profiling

1
1. scibot 20 Oct 2022
  
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  Addgene_44251
  
  DOI: 10.1016/j.crmeth.2022.100318
  
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sciencedirect.com/science/article/pii/S2667237522002089
Dec 2021
onlinelibrary.wiley.com onlinelibrary.wiley.com

CRISPRi enables fast growth followed by stable aerobic pyruvate formation in Escherichia coli without auxotrophy

1
1. scibot 01 Dec 2021
  
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  RRID:Addgene_44251
  
  DOI: 10.1002/elsc.202100021
  
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onlinelibrary.wiley.com/doi/10.1002/elsc.202100021
Aug 2021
www.sciencedirect.com www.sciencedirect.com

CRISPR-based peptide library display and programmable microarray self-assembly for rapid quantitative protein binding assays

1
1. scibot 16 Aug 2021
  
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  Addgene_44251
  
  DOI: 10.1016/j.molcel.2021.07.027
  
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sciencedirect.com/science/article/pii/S1097276521005967