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Cytotoxicity of activator expression in CRISPR-based transcriptional activation systems

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1. scibot 03 Sep 2025
  
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  DOI: 10.1038/s41467-025-63570-4
  
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nature.com/articles/s41467-025-63570-4
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Endogenous fine-mapping and prioritization of functional regulatory elements in complex genetic loci

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  DOI: 10.1016/j.xgen.2025.100982
  
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cell.com/cell-genomics/abstract/S2666-979X(25)00238-1
Aug 2025
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Membrane-wide screening identifies potential tissue-specific determinants of SARS-CoV-2 tropism

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pmc.ncbi.nlm.nih.gov/articles/PMC12286382/
Jul 2025
www.medrxiv.org www.medrxiv.org

An integrated framework for functional dissection of variable expressivity in genetic disorders

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1. scibot 30 Jul 2025
  
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  DOI: 10.1101/2025.07.22.25331885
  
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medrxiv.org/content/10.1101/2025.07.22.25331885v2
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Prion-induced ferroptosis is facilitated by RAC3

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pmc.ncbi.nlm.nih.gov/articles/PMC12198409/
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Near-infrared light activatable chemically induced CRISPR system

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1. scibot 20 Jul 2025
  
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pmc.ncbi.nlm.nih.gov/articles/PMC12214668/
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Leveraging CRISPR activation for rapid assessment of gene editing products in human pluripotent stem cells

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1. scibot 20 Jul 2025
  
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pmc.ncbi.nlm.nih.gov/articles/PMC12181958/
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An alternate receptor for adeno-associated viruses

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sciencedirect.com/science/article/pii/S0092867425006920
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Requirement for Cyclin D1 Underlies Cell-Autonomous HIF2 Dependence in Kidney Cancer

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pmc.ncbi.nlm.nih.gov/articles/PMC12223508/
Jun 2025
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Systemic genome-epigenome analysis captures a lineage-specific super-enhancer for MYB in gastrointestinal adenocarcinoma

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1. scibot 15 Jun 2025
  
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pmc.ncbi.nlm.nih.gov/articles/PMC12130324/
May 2025
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Integrative characterization of MYC RNA-binding function

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sciencedirect.com/science/article/pii/S2666979X2500134X
Apr 2025
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Identification of a SNAI1 enhancer RNA that drives cancer cell plasticity

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pmc.ncbi.nlm.nih.gov/articles/PMC11937597/
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Modified screening of MYC promotor region elements using the CRISPR library in ovarian cancer

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1. scibot 05 Apr 2025
  
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pmc.ncbi.nlm.nih.gov/articles/PMC11963443/
Mar 2025
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Amplified dosage of the NKX2-1 lineage transcription factor controls its oncogenic role in lung adenocarcinoma

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1. scibot 29 Mar 2025
  
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  DOI: 10.1016/j.molcel.2025.03.001
  
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sciencedirect.com/science/article/pii/S1097276525001947
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Circulating tumor cell plasticity determines breast cancer therapy resistance via neuregulin 1–HER3 signaling

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  RRID:Addgene_61425 DOI:10.1038/s43018-024-00882-2
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pmc.ncbi.nlm.nih.gov/articles/PMC11779641/
Feb 2025
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Development of cell lines with increased susceptibility to diverse adeno-associated viral vectors to enable in vitro potency assays

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  DOI: 10.1016/j.omtm.2025.101416
  
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sciencedirect.com/science/article/pii/S2329050125000117
Dec 2024
genomebiology.biomedcentral.com genomebiology.biomedcentral.com

Functional screening reveals genetic dependencies and diverging cell cycle control in atypical teratoid rhabdoid tumors

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1. scibot 05 Dec 2024
  
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genomebiology.biomedcentral.com/articles/10.1186/s13059-024-03438-w
Sep 2024
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The proteogenomic landscape of multiple myeloma reveals insights into disease biology and therapeutic opportunities

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1. scibot 29 Sep 2024
  
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  DOI: 10.1038/s43018-024-00784-3
  
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ncbi.nlm.nih.gov/pmc/articles/PMC11358022/
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Cytotoxicity of Activator Expression in CRISPR-based Transcriptional Activation Systems

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1. scibot 27 Sep 2024
  
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  DOI: 10.1101/2024.09.23.614524
  
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biorxiv.org/content/10.1101/2024.09.23.614524v1
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Evolved cytidine and adenine base editors with high precision and minimized off-target activity by a continuous directed evolution system in mammalian cells

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1. scibot 25 Sep 2024
  
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  DOI: 10.1038/s41467-024-52483-3
  
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ncbi.nlm.nih.gov/pmc/articles/PMC11408606/
Aug 2024
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Lecithin-cholesterol acyltransferase is a potential tumor suppressor and predictive marker for hepatocellular carcinoma metastasis

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1. scibot 26 Aug 2024
  
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  61425
  
  DOI: 10.4251/wjgo.v16.i8.3651
  
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ncbi.nlm.nih.gov/pmc/articles/PMC11334038/
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Crosstalk of MAP3K1 and EGFR signaling mediates gene-environment interactions that block developmental tissue closure

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  DOI: 10.1016/j.jbc.2024.107486
  
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ncbi.nlm.nih.gov/pmc/articles/PMC11294703/
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Convergence of coronary artery disease genes onto endothelial cell programs

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  DOI: 10.1038/s41586-024-07022-x
  
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ncbi.nlm.nih.gov/pmc/articles/PMC10921916/
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Epigenomic signatures of sarcomatoid differentiation to guide the treatment of renal cell carcinoma

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cell.com/cell-reports/fulltext/S2211-1247(24)00678-8
Jun 2024
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CRISPR/Cas9 screening reveals a distinct class of MHC-I binders with precise HLA-peptide recognition

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1. scibot 30 Jun 2024
  
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ncbi.nlm.nih.gov/pmc/articles/PMC11209011/
May 2024
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Conversion of natural cytokine receptors into orthogonal synthetic biosensors

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1. scibot 25 May 2024
  
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  plasmid_61425
  
  DOI: 10.1101/2024.03.23.586421
  
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biorxiv.org/content/10.1101/2024.03.23.586421v1
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CRISPR-Cas13d screens identify KILR, a breast cancer risk-associated lncRNA that regulates DNA replication and repair

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1. scibot 19 May 2024
  
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  Addgene_61
  
  DOI: 10.1186/s12943-024-02021-y
  
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ncbi.nlm.nih.gov/pmc/articles/PMC11094906/
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Mapping of DDX11 genetic interactions defines sister chromatid cohesion as the major dependency

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ncbi.nlm.nih.gov/pmc/articles/PMC11075568/
Apr 2024
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Genome-wide CRISPR screens identify the YAP/TEAD axis as a driver of persister cells in EGFR mutant lung cancer

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1. scibot 30 Apr 2024
  
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  DOI: 10.1038/s42003-024-06190-w
  
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ncbi.nlm.nih.gov/pmc/articles/PMC11043391/
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Loss of NEDD8 in cancer cells causes vulnerability to immune checkpoint blockade in triple-negative breast cancer

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1. scibot 30 Apr 2024
  
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ncbi.nlm.nih.gov/pmc/articles/PMC11055868/
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Long noncoding RNA Malat1 protects against osteoporosis and bone metastasis

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  DOI: 10.1038/s41467-024-46602-3
  
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ncbi.nlm.nih.gov/pmc/articles/PMC10944492/
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MAP3K1 regulates female reproductive tract development

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1. scibot 14 Apr 2024
  
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  #61425
  
  DOI: 10.1242/dmm.050669
  
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ncbi.nlm.nih.gov/pmc/articles/PMC10985838/
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Genome-wide CRISPR activation screen identifies JADE3 as an antiviral activator of NF-kB–dependent IFITM3 expression

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  Addgene_61425
  
  DOI: 10.1016/j.jbc.2024.107153
  
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ncbi.nlm.nih.gov/pmc/articles/PMC11001640/
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Tumor cell-intrinsic epigenetic dysregulation shapes cancer-associated fibroblasts heterogeneity to metabolically support pancreatic cancer

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  DOI: 10.1016/j.ccell.2024.03.005
  
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sciencedirect.com/science/article/pii/S1535610824000904
Mar 2024
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Functionally-instructed modifiers of response to ATR inhibition in experimental glioma

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1. scibot 15 Mar 2024
  
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  RRID:Addgene_61425
  
  DOI: 10.1186/s13046-024-02995-z
  
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ncbi.nlm.nih.gov/pmc/articles/PMC10935927/
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CDK9-55 guides the anaphase-promoting complex/cyclosome (APC/C) in choosing the DNA repair pathway choice

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1. scibot 14 Mar 2024
  
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  RRID:Addgene_61425
  
  DOI: 10.1038/s41388-024-02982-w
  
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nature.com/articles/s41388-024-02982-w
Feb 2024
www.ncbi.nlm.nih.gov www.ncbi.nlm.nih.gov

Sequential CRISPR screening reveals partial NatB inhibition as a strategy to mitigate alpha-synuclein levels in human neurons

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1. scibot 15 Feb 2024
  
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  plasmid_61425
  
  DOI: 10.1126/sciadv.adj4767
  
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ncbi.nlm.nih.gov/pmc/articles/PMC10857481/
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Genome-wide quantification of copy-number aberration impact on gene expression in ovarian high-grade serous carcinoma

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1. scibot 08 Feb 2024
  
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  plasmid_61
  
  DOI: 10.1186/s12885-024-11895-6
  
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ncbi.nlm.nih.gov/pmc/articles/PMC10840274/
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Redox signalling regulates breast cancer metastasis via phenotypic and metabolic reprogramming due to p63 activation by HIF1α

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Dec 2023
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The MHC-E peptide ligands for checkpoint CD94/NKG2A are governed by inflammatory signals, whereas LILRB1/2 receptors are peptide indifferent

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Single-cell functional genomics reveals determinants of sensitivity and resistance to natural killer cells in blood cancers

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Differences in syncytia formation by SARS-CoV-2 variants modify host chromatin accessibility and cellular senescence via TP53

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LINE-1 activation in the cerebellum drives ataxia

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Differential Modulation of Polycomb-Associated Histone Marks by cBAF, pBAF, and gBAF Complexes

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biorxiv.org/content/10.1101/2023.09.23.557848v2
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STAT1 is required to establish but not maintain interferon-γ-induced transcriptional memory

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embopress.org/doi/10.15252/embj.2022112259
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Single-cell profiling of lncRNAs in human germ cells and molecular analysis reveals transcriptional regulation of LNC1845 on LHX8

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Tumor-intrinsic SIRPA promotes sensitivity to checkpoint inhibition immunotherapy in melanoma

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Acute depletion of human core nucleoporin reveals direct roles in transcription control but dispensability for 3D genome organization

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Oct 2022
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ZBTB7A promotes virus-host homeostasis during human coronavirus 229E infection

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Aug 2022
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CRISPR interference interrogation of COPD GWAS genes reveals the functional significance of desmoplakin in iPSC-derived alveolar epithelial cells

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CRISPR interference interrogation of COPD GWAS genes reveals the functional significance of desmoplakin in iPSC-derived alveolar epithelial cells

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Apr 2022
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Reduced mitochondrial fusion and Huntingtin levels contribute to impaired dendritic maturation and behavioral deficits in Fmr1 mutant mice

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ncbi.nlm.nih.gov/pmc/articles/PMC6556892/
Feb 2022
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HiCAR is a robust and sensitive method to analyze open-chromatin-associated genome organization

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  DOI: 10.1016/j.molcel.2022.01.023
  
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Inflammatory fibroblasts mediate resistance to neoadjuvant therapy in rectal cancer

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Upregulation of CD14 in mesenchymal stromal cells accelerates lipopolysaccharide-induced response and enhances antibacterial properties

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sciencedirect.com/science/article/pii/S2589004222000293
Oct 2021
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A bipartite element with allele-specific functions safeguards DNA methylation imprints at the Dlk1-Dio3 locus

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AT-rich interaction domain 5A regulates the transcription of interleukin-6 gene in prostate cancer cells

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onlinelibrary.wiley.com/doi/10.1002/pros.24251
Aug 2021
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Enhancer RNA m6A methylation facilitates transcriptional condensate formation and gene activation

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Untitled document

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  DOI: 10.1016/j.stem.2019.03.012
  
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Jul 2021
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Epigenetic Silencing of CDR1as Drives IGF2BP3-Mediated Melanoma Invasion and Metastasis

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BAP1/ASXL complex modulation regulates epithelial-mesenchymal transition during trophoblast differentiation and invasion

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elifesciences.org/articles/63254
May 2021
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Lipolysis drives expression of the constitutively active receptor GPR3 to induce adipose thermogenesis

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sciencedirect.com/science/article/pii/S0092867421005729
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Pooled CRISPR-activation screening coupled with single-cell RNA-seq in mouse embryonic stem cells

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  DOI: 10.1016/j.xpro.2021.100426
  
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Feb 2021
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Hominoid-Specific Transposable Elements and KZFPs Facilitate Human Embryonic Genome Activation and Control Transcription in Naive Human ESCs

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cell.com/cell-stem-cell/abstract/S1934-5909(19)30111-0
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Epigenetic Silencing of CDR1as Drives IGF2BP3-Mediated Melanoma Invasion and Metastasis

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sciencedirect.com/science/article/pii/S153561081930577X
Jan 2021
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Functional Genomics Identify Distinct and Overlapping Genes Mediating Resistance to Different Classes of Heterobifunctional Degraders of Oncoproteins

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sciencedirect.com/science/article/pii/S2211124720315217
Sep 2020
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Epigenetic Silencing of CDR1as Drives IGF2BP3-Mediated Melanoma Invasion and Metastasis

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  RRID:Addgene_61425
  
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DUX-miR-344-ZMYM2-Mediated Activation of MERVL LTRs Induces a Totipotent 2C-like State

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The COMET toolkit for composing customizable genetic programs in mammalian cells

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ncbi.nlm.nih.gov/pmc/articles/PMC7005830/
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A Single-Cell Transcriptomics CRISPR-Activation Screen Identifies Epigenetic Regulators of the Zygotic Genome Activation Program

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Super-Enhancer Redistribution as a Mechanism of Broad Gene Dysregulation in Repeatedly Drug-Treated Cancer Cells

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  DOI: 10.1016/j.celrep.2020.107532
  
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sciencedirect.com/science/article/pii/S2211124720304320
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Identification and Massively Parallel Characterization of Regulatory Elements Driving Neural Induction

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  DOI: 10.1016/j.stem.2019.09.010
  
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Untitled document

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nature.com/articles/s41593-019-0338-y

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