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Sep 2025
www.biorxiv.org www.biorxiv.org

Mechanistic insight into the regulation of virulence factor type 1 pili in pathogenic E. coli by rhomboid protease GlpG

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1. scibot 16 Sep 2025
  
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  Addgene: #62225
  
  DOI: 10.1101/2025.09.09.675128
  
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  Curator: @dhovakimyan1
  
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biorxiv.org/content/10.1101/2025.09.09.675128v1
Apr 2025
pmc.ncbi.nlm.nih.gov pmc.ncbi.nlm.nih.gov

Pamoic acid and carbenoxolone specifically inhibit CRISPR/Cas9 in bacteria, mammalian cells, and mice in a DNA topology-specific manner

2
1. scibot 07 Apr 2025
  
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  62,225
  
  DOI: 10.1186/s13059-025-03521-w
  
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2. scibot 07 Apr 2025
  
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  Addgene_62225
  
  DOI: 10.1186/s13059-025-03521-w
  
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pmc.ncbi.nlm.nih.gov/articles/PMC11951523/
pmc.ncbi.nlm.nih.gov pmc.ncbi.nlm.nih.gov

CnRed: Efficient, Marker-free Genome Engineering of Cupriavidus necator H16 by Adapted Lambda Red Recombineering

1
1. scibot 07 Apr 2025
  
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  Addgene_62225
  
  DOI: 10.1021/acssynbio.4c00757
  
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pmc.ncbi.nlm.nih.gov/articles/PMC11934132/
Mar 2025
pmc.ncbi.nlm.nih.gov pmc.ncbi.nlm.nih.gov

Activating the d-Tagatose Production Capacity of Escherichia coli with Structural Insights into C4 Epimerase Specificity

1
1. scibot 23 Mar 2025
  
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  addgene_62225
  
  DOI: 10.1021/acs.jafc.4c12842
  
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pmc.ncbi.nlm.nih.gov/articles/PMC11907403/
Feb 2025
pmc.ncbi.nlm.nih.gov pmc.ncbi.nlm.nih.gov

CRISPR/Cas9-mediated genomic insertion of functional genes into Lactiplantibacillus plantarum WCFS1

1
1. scibot 18 Feb 2025
  
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  pCas
  
  DOI: 10.1128/spectrum.02025-24
  
  Resource: RRID:Addgene_62225
  
  Curator: @olekpark
  
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pmc.ncbi.nlm.nih.gov/articles/PMC11792511/
pmc.ncbi.nlm.nih.gov pmc.ncbi.nlm.nih.gov

Cryptic genetic variation shapes the fate of gene duplicates in a protein interaction network

1
1. scibot 16 Feb 2025
  
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  pCAS
  
  DOI: 10.1038/s41467-025-56597-0
  
  Resource: RRID:Addgene_62225
  
  Curator: @olekpark
  
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pmc.ncbi.nlm.nih.gov/articles/PMC11814230/
Jul 2024
www.ncbi.nlm.nih.gov www.ncbi.nlm.nih.gov

Computer-aided design and implementation of efficient biosynthetic pathways to produce high added-value products derived from tyrosine in Escherichia coli

2
1. scibot 12 Jul 2024
  
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  RRID:Addgene_62225
  
  DOI: 10.3389/fbioe.2024.1360740
  
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2. scibot 12 Jul 2024
  
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  RRID:Addgene_62225
  
  DOI: 10.3389/fbioe.2024.1360740
  
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ncbi.nlm.nih.gov/pmc/articles/PMC11228882/
May 2024
www.ncbi.nlm.nih.gov www.ncbi.nlm.nih.gov

Precise microbiome engineering using natural and synthetic bacteriophages targeting an artificial bacterial consortium

1
1. scibot 19 May 2024
  
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  RRID:Addgene_62225
  
  DOI: 10.3389/fmicb.2024.1403903
  
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ncbi.nlm.nih.gov/pmc/articles/PMC11096457/
Apr 2024
www.ncbi.nlm.nih.gov www.ncbi.nlm.nih.gov

Cell-Free Protein Expression in Polymer Materials

1
1. scibot 30 Apr 2024
  
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  plasmid_62225
  
  DOI: 10.1021/acssynbio.3c00628
  
  Resource: RRID:Addgene_62225
  
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ncbi.nlm.nih.gov/pmc/articles/PMC11036507/
www.ncbi.nlm.nih.gov www.ncbi.nlm.nih.gov

The inactivation of tolC sensitizes Escherichia coli to perturbations in lipopolysaccharide transport

1
1. scibot 23 Apr 2024
  
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  62225
  
  DOI: 10.1016/j.isci.2024.109592
  
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ncbi.nlm.nih.gov/pmc/articles/PMC11019271/
Feb 2024
www.ncbi.nlm.nih.gov www.ncbi.nlm.nih.gov

Stable expression of HIV-1 MPER extended epitope on the surface of the recombinant probiotic bacteria Escherichia Coli Nissle 1917 using CRISPR/Cas9

1
1. scibot 08 Feb 2024
  
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  62,225
  
  DOI: 10.1186/s12934-023-02290-0
  
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  Curator: @olekpark
  
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ncbi.nlm.nih.gov/pmc/articles/PMC10840157/
Nov 2023
www.sciencedirect.com www.sciencedirect.com

Engineered bacteria titrate hydrogen sulfide and induce concentration-dependent effects on the host in a gut microphysiological system

1
1. scibot 28 Nov 2023
  
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  RRID:Addgene_62225
  
  DOI: 10.1016/j.celrep.2023.113481
  
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sciencedirect.com/science/article/pii/S2211124723014936
Feb 2023
journals.asm.org journals.asm.org

A Retro-Aldol Reaction Prompted the Evolvability of a Phosphotransferase System for the Utilization of a Rare Sugar

1
1. scibot 18 Feb 2023
  
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  RRID:Addgene_62225
  
  DOI: 10.1128/spectrum.03660-22
  
  Resource: RRID:Addgene_62225
  
  Curator: @scibot
  
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journals.asm.org/doi/10.1128/spectrum.03660-22
Aug 2021
www.sciencedirect.com www.sciencedirect.com

Synthetic biosensor accelerates evolution by rewiring carbon metabolism toward a specific metabolite

1
1. scibot 25 Aug 2021
  
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  Plasmid_62225
  
  DOI: 10.1016/j.celrep.2021.109589
  
  Resource: RRID:Addgene_62225
  
  Curator: @scibot
  
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sciencedirect.com/science/article/pii/S2211124721010238
www.ncbi.nlm.nih.gov www.ncbi.nlm.nih.gov

Structure of Escherichia coli respiratory complex I reconstituted into lipid nanodiscs reveals an uncoupled conformation

1
1. scibot 17 Aug 2021
  
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  RRID:Addgene_62225
  
  DOI: 10.7554/eLife.68710
  
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ncbi.nlm.nih.gov/pmc/articles/PMC8357420/
Jun 2021
www.sciencedirect.com www.sciencedirect.com

An acquired acyltransferase promotes Klebsiella pneumoniae ST258 respiratory infection

1
1. scibot 02 Jun 2021
  
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  Addgene_62225
  
  DOI: 10.1016/j.celrep.2021.109196
  
  Resource: RRID:Addgene_62225
  
  Curator: @Naa003
  
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sciencedirect.com/science/article/pii/S2211124721005428
Apr 2021
www.sciencedirect.com www.sciencedirect.com

Discovery of an Inhibitor for Bacterial 3-Mercaptopyruvate Sulfurtransferase that Synergistically Controls Bacterial Survival

1
1. scibot 28 Apr 2021
  
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  plasmid_62225
  
  DOI: 10.1016/j.chembiol.2020.10.012
  
  Resource: RRID:Addgene_62225
  
  Curator: @scibot
  
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sciencedirect.com/science/article/pii/S2451945620304281