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Protocol for iterative indirect immunofluorescence imaging of frozen mouse intestinal tissues

1
1. scibot 07 Oct 2026
  
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  Addgene plasmid: #85044
  
  DOI: 10.1016/j.xpro.2026.104410
  
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pmc.ncbi.nlm.nih.gov/articles/PMC12992094/
Aug 2026
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Synthetic multicolor antigen-stabilizable nanobody platform for intersectional labelling and functional imaging

1
1. scibot 09 Aug 2026
  
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  85044
  
  DOI: 10.1038/s41592-026-03056-3
  
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pmc.ncbi.nlm.nih.gov/articles/PMC13390901/
Feb 2026
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A Site-Specific Self-Association of a Protein Hub Drives Its Phase Separation

1
1. scibot 01 Feb 2026
  
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  Addgene_85044
  
  DOI: 10.1021/acschembio.5c00424
  
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pmc.ncbi.nlm.nih.gov/articles/PMC12813979/
Jan 2026
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A Reporter Platform to Study Therapy‐Induced Senescence in Live Cancer Cells

1
1. scibot 30 Jan 2026
  
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  plasmid_85
  
  DOI: 10.1002/smtd.202501270
  
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pmc.ncbi.nlm.nih.gov/articles/PMC12716209/
Nov 2025
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A Rho GTPase-effector ensemble governs cell migration behavior

1
1. scibot 24 Nov 2025
  
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  85044
  
  DOI: 10.1038/s41467-025-64635-0
  
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pmc.ncbi.nlm.nih.gov/articles/PMC12579213/
Sep 2025
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Generation of Knock‐In Syrian Hamsters via Zygote Microinjection Using CRISPR/Cas9 Genome Editing

1
1. scibot 22 Sep 2025
  
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  plasmid_85044
  
  DOI: 10.1002/dvg.70027
  
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pmc.ncbi.nlm.nih.gov/articles/PMC12444848/
www.biorxiv.org www.biorxiv.org

The axonal ER couples translation and secretion machineries for local delivery of axonal transmembrane proteins to promote axonal development

1
1. scibot 18 Sep 2025
  
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  plasmid_85044
  
  DOI: 10.1101/2025.09.09.674816
  
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biorxiv.org/content/10.1101/2025.09.09.674816v1
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Mitochondrial proteostatic stress disrupts mitoribosome biogenesis and translation

1
1. scibot 18 Sep 2025
  
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  RRID:Addgene_85044
  
  DOI: 10.1101/2025.09.05.673478
  
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biorxiv.org/content/10.1101/2025.09.05.673478v1
Jul 2025
pmc.ncbi.nlm.nih.gov pmc.ncbi.nlm.nih.gov

Cellular and biophysical barriers to lipid nanoparticle mediated delivery of RNA to the cytosol

1
1. scibot 20 Jul 2025
  
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  plasmid_85044
  
  DOI: 10.1038/s41467-025-60959-z
  
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pmc.ncbi.nlm.nih.gov/articles/PMC12217163/
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Genetically encoded affinity reagents are a toolkit for visualizing and manipulating endogenous protein function in vivo

1
1. scibot 04 Jul 2025
  
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  RRID:Addgene_85044
  
  DOI: 10.1038/s41467-025-61003-w
  
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pmc.ncbi.nlm.nih.gov/articles/PMC12216260/
Jun 2025
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Efficient CRISPR/Cas9‐mediated knockin of reporter genes in rats at ROSA26 by pronuclear microinjection

1
1. scibot 15 Jun 2025
  
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  plasmid_85044
  
  DOI: 10.1111/dgd.70007
  
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pmc.ncbi.nlm.nih.gov/articles/PMC12099484/
May 2025
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Weight Gain With Advancing Age Is Controlled by the Muscarinic Acetylcholine Receptor M4 in Male Mice

1
1. scibot 16 May 2025
  
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  85044
  
  DOI: 10.1210/endocr/bqaf064
  
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pmc.ncbi.nlm.nih.gov/articles/PMC12012353/
Apr 2025
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The nucleoid of rapidly growing Escherichia coli localizes close to the inner membrane and is organized by transcription, translation, and cell geometry

1
1. scibot 24 Apr 2025
  
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  RRID:Addgene_85044
  
  DOI: 10.1038/s41467-025-58723-4
  
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pmc.ncbi.nlm.nih.gov/articles/PMC12009437/
Mar 2025
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Acute diacylglycerol production activates critical membrane-shaping proteins leading to mitochondrial tubulation and fission

1
1. scibot 23 Mar 2025
  
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  Plasmid_85044
  
  DOI: 10.1038/s41467-025-57439-9
  
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pmc.ncbi.nlm.nih.gov/articles/PMC11920102/
Feb 2025
advanced.onlinelibrary.wiley.com advanced.onlinelibrary.wiley.com

An Injection-Molded Modified Silicone Rubber for Cancer-on-Chip Applications

1
1. scibot 13 Feb 2025
  
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  RRID:Addgene_85
  
  DOI: 10.1002/admt.202401450
  
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  Curator: @inessasarian
  
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  RRID:Addgene_85044 RRIDCUR:Unresolved
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advanced.onlinelibrary.wiley.com/doi/10.1002/admt.202401450
Jul 2024
www.nature.com www.nature.com

Actomyosin organelle functions of SPIRE actin nucleators precede animal evolution

1
1. scibot 11 Jul 2024
  
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  RRID:Addgene_85044
  
  DOI: 10.1038/s42003-024-06458-1
  
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nature.com/articles/s42003-024-06458-1
May 2024
www.biorxiv.org www.biorxiv.org

Spatiotemporal proteomics reveals the biosynthetic lysosomal membrane protein interactome in neurons

1
1. scibot 23 May 2024
  
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  plasmid_85044
  
  DOI: 10.1101/2024.05.16.594502
  
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biorxiv.org/content/10.1101/2024.05.16.594502v1
www.ncbi.nlm.nih.gov www.ncbi.nlm.nih.gov

Development of rhesus macaque astrocyte cell lines supporting infection with a panel of viruses

1
1. scibot 19 May 2024
  
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  plasmid_85044
  
  DOI: 10.1371/journal.pone.0303059
  
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ncbi.nlm.nih.gov/pmc/articles/PMC11093292/
Apr 2024
www.ncbi.nlm.nih.gov www.ncbi.nlm.nih.gov

KIS counteracts PTBP2 and regulates alternative exon usage in neurons

1
1. scibot 30 Apr 2024
  
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  85044
  
  DOI: 10.7554/eLife.96048
  
  Resource: RRID:Addgene_85044
  
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ncbi.nlm.nih.gov/pmc/articles/PMC11045219/
www.ncbi.nlm.nih.gov www.ncbi.nlm.nih.gov

Persistent TFIIH binding to non-excised DNA damage causes cell and developmental failure

1
1. scibot 29 Apr 2024
  
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  plasmid_85044
  
  DOI: 10.1038/s41467-024-47935-9
  
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ncbi.nlm.nih.gov/pmc/articles/PMC11045817/
www.ncbi.nlm.nih.gov www.ncbi.nlm.nih.gov

Rab11 regulates autophagy at dendritic spines in an mTOR- and NMDA-dependent manner

1
1. scibot 23 Apr 2024
  
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  RRID:Addgene_85044
  
  DOI: 10.1091/mbc.E23-02-0060
  
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ncbi.nlm.nih.gov/pmc/articles/PMC10916872/
Feb 2024
www.ncbi.nlm.nih.gov www.ncbi.nlm.nih.gov

DENND6A links Arl8b to a Rab34/RILP/dynein complex, regulating lysosomal positioning and autophagy

1
1. scibot 05 Feb 2024
  
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  85044
  
  DOI: 10.1038/s41467-024-44957-1
  
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ncbi.nlm.nih.gov/pmc/articles/PMC10830484/
Dec 2023
www.biorxiv.org www.biorxiv.org

Genetically encoded affinity reagents (GEARs): A toolkit for visualizing and manipulating endogenous protein function in vivo

1
1. scibot 01 Dec 2023
  
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  RRID:Addgene_85044
  
  DOI: 10.1101/2023.11.15.567075
  
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biorxiv.org/content/10.1101/2023.11.15.567075v1
Oct 2023
www.biorxiv.org www.biorxiv.org

Transertion and cell geometry organize the Escherichia coli nucleoid during rapid growth

1
1. scibot 27 Oct 2023
  
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  RRID:Addgene_85044
  
  DOI: 10.1101/2023.10.16.562172
  
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biorxiv.org/content/10.1101/2023.10.16.562172v2
dx.doi.org dx.doi.org

Live-cell imaging unveils distinct R-loop populations with heterogeneous dynamics

1
1. scibot 13 Oct 2023
  
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  RRID:Addgene_85044
  
  DOI: 10.1093/nar/gkad812
  
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dx.doi.org/10.1093/nar/gkad812
Aug 2023
www.ncbi.nlm.nih.gov www.ncbi.nlm.nih.gov

Cas phosphorylation regulates focal adhesion assembly

1
1. scibot 26 Aug 2023
  
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  plasmid_85044
  
  DOI: 10.7554/eLife.90234
  
  Resource: RRID:Addgene_85044
  
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ncbi.nlm.nih.gov/pmc/articles/PMC10435235/
Nov 2022
www.ncbi.nlm.nih.gov www.ncbi.nlm.nih.gov

A role for the centrosome in regulating the rate of neuronal efferocytosis by microglia in vivo

1
1. scibot 24 Nov 2022
  
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  plasmid_85044
  
  DOI: 10.7554/eLife.82094
  
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ncbi.nlm.nih.gov/pmc/articles/PMC9674339/
www.sciencedirect.com www.sciencedirect.com

Development of single-molecule ubiquitination mediated fluorescence complementation to visualize protein ubiquitination dynamics in dendrites

1
1. scibot 16 Nov 2022
  
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  RRID:Addgene_85044
  
  DOI: 10.1016/j.celrep.2022.111658
  
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sciencedirect.com/science/article/pii/S2211124722015297
Mar 2022
www.sciencedirect.com www.sciencedirect.com

Force-generating apoptotic cells orchestrate avian neural tube bending

1
1. scibot 18 Mar 2022
  
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  RRID:Addgene_85044
  
  DOI: 10.1016/j.devcel.2022.02.020
  
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sciencedirect.com/science/article/pii/S1534580722001228
Dec 2021
www.sciencedirect.com www.sciencedirect.com

A mechanistic basis for the malignant progression of salivary gland tumors

1
1. scibot 08 Dec 2021
  
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  Addgene_85044
  
  DOI: 10.1016/j.isci.2021.103508
  
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sciencedirect.com/science/article/pii/S2589004221014796
Aug 2021
elifesciences.org elifesciences.org

Quantitative live-cell imaging and computational modeling shed new light on endogenous WNT/CTNNB1 signaling dynamics

1
1. scibot 15 Aug 2021
  
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  RRID:Addgene_85044
  
  DOI: 10.7554/eLife.66440
  
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elifesciences.org/articles/66440
journals.plos.org journals.plos.org

Untitled document

1
1. scibot 12 Aug 2021
  
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  plasmid_85044
  
  DOI: 10.1371/journal.pone.0212757
  
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journals.plos.org/plosone/article
May 2021
www.sciencedirect.com www.sciencedirect.com

Rab34 GTPase mediates ciliary membrane formation in the intracellular ciliogenesis pathway

1
1. scibot 14 May 2021
  
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  Addgene_85044
  
  DOI: 10.1016/j.cub.2021.04.075
  
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sciencedirect.com/science/article/pii/S0960982221006229
www.nature.com www.nature.com

Co-occupancy identifies transcription factor co-operation for axon growth

1
1. scibot 12 May 2021
  
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  RRID:Addgene_85044
  
  DOI: 10.1038/s41467-021-22828-3
  
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nature.com/articles/s41467-021-22828-3
Feb 2021
www.ncbi.nlm.nih.gov www.ncbi.nlm.nih.gov

EphrinB2 clustering by Nipah virus G is required to activate and trap F intermediates at supported lipid bilayer–cell interfaces

1
1. scibot 09 Feb 2021
  
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  RRID:Addgene_85044
  
  DOI: 10.1126/sciadv.abe1235
  
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  RRID:Addgene_85044 RRIDCUR:Validated
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ncbi.nlm.nih.gov/pmc/articles/PMC7840137/
Sep 2020
www.cell.com www.cell.com

Importin α5 Regulates Anxiety through MeCP2 and Sphingosine Kinase 1

1
1. scibot 26 Sep 2020
  
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  plasmid_85044
  
  DOI: 10.1016/j.celrep.2018.11.066
  
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  Curator: @evieth
  
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  RRIDCUR:Validated RRID:Addgene_85044
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cell.com/cell-reports/abstract/S2211-1247(18)31842-4
Jan 2020
europepmc.org europepmc.org

Untitled document

1
1. scibot 12 Jan 2020
  
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  plasmid_85044
  
  DOI: 10.1016/j.celrep.2018.11.066
  
  Resource: RRID:Addgene_85044
  
  Curator: @evieth
  
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  RRID:Addgene_85044 RRIDCUR:Validated
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europepmc.org/articles/PMC6302549/
www.sciencedirect.com www.sciencedirect.com

Untitled document

1
1. scibot 12 Jan 2020
  
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  plasmid_85044
  
  DOI: 10.1016/j.celrep.2018.11.066
  
  Resource: RRID:Addgene_85044
  
  Curator: @evieth
  
  SciCrunch record: plasmid_85044
  
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  RRID:Addgene_85044 RRIDCUR:Validated
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Tags

RRIDCUR:Validated

RRID:Addgene_85044

Annotators

scibot

URL

sciencedirect.com/science/article/pii/S2211124718318424