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Aug 2025
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PERRC: Protease Engineering with Reactant Residence Time Control

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1. scibot 11 Aug 2025
  
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  DOI: 10.1021/acssynbio.5c00154
  
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pmc.ncbi.nlm.nih.gov/articles/PMC12301959/
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A New Highly Specific and Soluble Protease for Precise Removal of N‑Terminal Purification Tags

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1. scibot 11 Aug 2025
  
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  DOI: 10.1021/acsomega.5c01764
  
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pmc.ncbi.nlm.nih.gov/articles/PMC12290709/
Jul 2025
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Regulatory mechanisms of PP2A complex assembly driven by physicochemical differences in A-subunit isoforms

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1. scibot 25 Jul 2025
  
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  DOI: 10.1016/j.str.2025.06.013
  
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sciencedirect.com/science/article/pii/S0969212625002485
Jun 2025
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Reconstruction of the ancient cyanobacterial proto-circadian clock system KaiABC

1
1. scibot 15 Jun 2025
  
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  DOI: 10.1038/s44318-025-00425-0
  
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pmc.ncbi.nlm.nih.gov/articles/PMC12084410/
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Engineering and Structural Elucidation of a Sac7d‐Derived IgG Fc‐Specific Affitin and Its Application for the Light‐Controlled Affinity Purification of Antibodies

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1. scibot 15 Jun 2025
  
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pmc.ncbi.nlm.nih.gov/articles/PMC12135134/
Apr 2025
52.53.155.43 52.53.155.43

An in vitro one pot synthetic biology approach to emulating Golgi O-glycosylation divergence in MUC1 and tumor associated MUC1

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1. scibot 24 Apr 2025
  
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  plasmid_8827
  
  DOI: 10.26434/chemrxiv-2024-bq9ct
  
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52.53.155.43/5e97e58133d27aed9149b07ac954ee51c94f16d5/an-in-vitro-one-pot-synthetic-biology-approach-to-emulating-golgi-o-glycosylation-divergence-in-muc1-and-tumor-associated-muc1-html.html
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PERRC: Protease Engineering with Reactant Residence Time Control

1
1. scibot 07 Apr 2025
  
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  Addgene_8827
  
  DOI: 10.1101/2025.03.02.641063
  
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pmc.ncbi.nlm.nih.gov/articles/PMC11908129/
Mar 2025
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Short 5’ UTRs serve as a marker for viral mRNA translation inhibition by the IFIT2-IFIT3 antiviral complex

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1. scibot 11 Mar 2025
  
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  DOI: 10.1101/2025.02.11.637299
  
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pmc.ncbi.nlm.nih.gov/articles/PMC11844544/
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Establishment of a Novel Caco‐2‐Based Cell Culture System for Human Sapovirus Propagation

1
1. scibot 01 Mar 2025
  
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  DOI: 10.1111/gtc.70007
  
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pmc.ncbi.nlm.nih.gov/articles/PMC11861566/
Feb 2025
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AcrVIB1 inhibits CRISPR-Cas13b immunity by promoting unproductive crRNA binding accessible to RNase attack

1
1. scibot 20 Feb 2025
  
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  Plasmid_8827
  
  DOI: 10.1016/j.molcel.2025.01.020
  
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sciencedirect.com/science/article/pii/S1097276525000541
Dec 2024
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PDF-html.html

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1. scibot 29 Dec 2024
  
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  plasmid_8827
  
  DOI: 10.26434/chemrxiv-2024-bq9ct-v2
  
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52.53.155.43/69f7d796c960110bf10609a29bd844a5b20a71e0/PDF-html.html
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G3BP1 promotes intermolecular RNA-RNA interactions during RNA condensation

1
1. scibot 24 Dec 2024
  
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  DOI: 10.1016/j.molcel.2024.11.012
  
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sciencedirect.com/science/article/pii/S1097276524009171
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Kunitz-type trypsin inhibitor from durian (Durio zibethinus) employs a distinct loop for trypsin inhibition

1
1. scibot 13 Dec 2024
  
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  RRID:Addgene_8827
  
  DOI: 10.1002/pro.5230
  
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onlinelibrary.wiley.com/doi/10.1002/pro.5230
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Assembly and Cryo-EM structure determination of yeast mitochondrial RNA polymerase initiation complex intermediates

1
1. scibot 03 Dec 2024
  
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  plasmid_8827
  
  DOI: 10.1016/j.xpro.2021.100431
  
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sciencedirect.com/science/article/pii/S2666166721001386
Nov 2024
pubs.acs.org pubs.acs.org

Universal Glycosyltransferase Continuous Assay for Uniform Kinetics and Inhibition Database Development and Mechanistic Studies Illustrated on ST3GAL1, C1GALT1, and FUT1

1
1. scibot 29 Nov 2024
  
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  DOI: 10.1021/acsomega.4c00485
  
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pubs.acs.org/doi/full/10.1021/acsomega.4c00485
Jun 2024
journals.asm.org journals.asm.org

Structural analyses of the GI.4 norovirus by cryo-electron microscopy and X-ray crystallography revealing binding sites for human monoclonal antibodies

2
1. scibot 06 Jun 2024
  
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  plasmid_8827
  
  DOI: 10.1128/jvi.00197-24
  
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  DOI: 10.1128/jvi.00197-24
  
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journals.asm.org/doi/10.1128/jvi.00197-24
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Proline Isomerization and Molten Globular Property of TgPDCD5 Secreted from Toxoplasma gondii Confers Its Regulation of Heparin Sulfate Binding

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1. scibot 03 Jun 2024
  
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  DOI: 10.1021/jacsau.3c00577
  
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ncbi.nlm.nih.gov/pmc/articles/PMC11134355/
May 2024
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Structural analyses of the GI.4 norovirus by cryo-electron microscopy and X-ray crystallography revealing binding sites for human monoclonal antibodies

1
1. scibot 19 May 2024
  
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  plasmid_8827
  
  DOI: 10.1128/jvi.00197-24
  
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ncbi.nlm.nih.gov/pmc/articles/PMC11092324/
52.53.155.43 52.53.155.43

Modular and integrative activity reporters enhance biochemical studies in the yeast ER

1
1. scibot 09 May 2024
  
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  RRID:Addgene_8827
  
  DOI: 10.1093/protein/gzae008
  
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52.53.155.43/3e27d81f137f55ff265e7a1c4a05d993e576eabb/gzae008-html.html
Apr 2024
www.ncbi.nlm.nih.gov www.ncbi.nlm.nih.gov

Harnessing noncanonical crRNAs to improve functionality of Cas12a orthologs

1
1. scibot 30 Apr 2024
  
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  Addgene_8827
  
  DOI: 10.1016/j.celrep.2024.113777
  
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ncbi.nlm.nih.gov/pmc/articles/PMC11031708/
pubs.acs.org pubs.acs.org

Universal Glycosyltransferase Continuous Assay for Uniform Kinetics and Inhibition Database Development and Mechanistic Studies Illustrated on ST3GAL1, C1GALT1, and FUT1

1
1. scibot 20 Apr 2024
  
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  RRID:Addgene_8827
  
  DOI: 10.1021/acsomega.4c00485
  
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pubs.acs.org/doi/full/10.1021/acsomega.4c00485
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A temperature-inducible protein module for control of mammalian cell fate

1
1. scibot 15 Apr 2024
  
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  Plasmid_8827
  
  DOI: 10.1101/2024.02.19.581019
  
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ncbi.nlm.nih.gov/pmc/articles/PMC10925237/
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Glutamylation of Npm2 and Nap1 acidic disordered regions increases DNA mimicry and histone chaperone efficiency

1
1. scibot 11 Apr 2024
  
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  DOI: 10.1016/j.isci.2024.109458
  
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ncbi.nlm.nih.gov/pmc/articles/PMC10987829/
Mar 2024
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The P granule antibody KT3 recognizes epitopes in both PGL-1 and PGL-3

1
1. scibot 08 Mar 2024
  
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  PRK793
  
  DOI: 10.17912/micropub.biology.001137
  
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ncbi.nlm.nih.gov/pmc/articles/PMC10910292/
Feb 2024
www.ncbi.nlm.nih.gov www.ncbi.nlm.nih.gov

Optimizing protein delivery rate from silk fibroin hydrogel using silk fibroin-mimetic peptides conjugation

1
1. scibot 28 Feb 2024
  
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  DOI: 10.1038/s41598-024-53689-7
  
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ncbi.nlm.nih.gov/pmc/articles/PMC10891107/
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The pathogen-encoded signalling receptor Tir exploits host-like intrinsic disorder for infection

1
1. scibot 21 Feb 2024
  
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  pRK793
  
  DOI: 10.1038/s42003-024-05856-9
  
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ncbi.nlm.nih.gov/pmc/articles/PMC10864410/
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Cullin-RING ligases employ geometrically optimized catalytic partners for substrate targeting

1
1. scibot 21 Feb 2024
  
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  RRID:Addgene_8827
  
  DOI: 10.1016/j.molcel.2024.01.022
  
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sciencedirect.com/science/article/pii/S1097276524000893
Nov 2023
www.ncbi.nlm.nih.gov www.ncbi.nlm.nih.gov

Dynamical control enables the formation of demixed biomolecular condensates

1
1. scibot 30 Nov 2023
  
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  DOI: 10.1038/s41467-023-43489-4
  
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ncbi.nlm.nih.gov/pmc/articles/PMC10667521/
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Deep mutational scanning highlights a role for cytosolic regions in Hrd1 function

1
1. scibot 28 Nov 2023
  
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  DOI: 10.1016/j.celrep.2023.113451
  
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sciencedirect.com/science/article/pii/S2211124723014638
Jun 2023
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Integrated regulation of tubulin tyrosination and microtubule stability by human α-tubulin isotypes

1
1. scibot 23 Jun 2023
  
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  DOI: 10.1016/j.celrep.2023.112653
  
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sciencedirect.com/science/article/pii/S2211124723006642
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Histone H3 lysine 27 crotonylation mediates gene transcriptional repression in chromatin

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1. scibot 23 Jun 2023
  
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  Plasmid_8827
  
  DOI: 10.1016/j.molcel.2023.05.022
  
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sciencedirect.com/science/article/pii/S1097276523003787
Feb 2023
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An enzymatic fluorimetric assay for determination of N-acetylaspartate

1
1. scibot 18 Feb 2023
  
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  DOI: 10.1016/j.ab.2023.115083
  
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sciencedirect.com/science/article/pii/S0003269723000489
May 2022
portlandpress.com portlandpress.com

Citrylglutamate synthase deficient male mice are subfertile with impaired histone and transition protein 2 removal in late spermatids

1
1. scibot 27 May 2022
  
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  RRID:Addgene_8827
  
  DOI: 10.1042/BCJ20210844
  
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portlandpress.com/biochemj/article/479/9/953/231167/Citrylglutamate-synthase-deficient-male-mice-are
Feb 2022
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Poxviruses and paramyxoviruses use a conserved mechanism of STAT1 antagonism to inhibit interferon signaling

1
1. scibot 23 Feb 2022
  
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  Addgene_8827
  
  DOI: 10.1016/j.chom.2022.01.014
  
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sciencedirect.com/science/article/pii/S1931312822000506
www.nature.com www.nature.com

Amyloid conformation-dependent disaggregation in a reconstituted yeast prion system

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1. scibot 22 Feb 2022
  
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  DOI: 10.1038/s41589-021-00951-y
  
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nature.com/articles/s41589-021-00951-y
Jan 2022
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Compartmentalization of telomeres through DNA-scaffolded phase separation

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1. scibot 26 Jan 2022
  
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  Addgene_8827
  
  DOI: 10.1016/j.devcel.2021.12.017
  
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sciencedirect.com/science/article/pii/S1534580721010376
Oct 2021
www.sciencedirect.com www.sciencedirect.com

Unlocking SARS-CoV-2 detection in low- and middle-income countries

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1. scibot 22 Oct 2021
  
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  RRID:Addgene_8827
  
  DOI: 10.1016/j.crmeth.2021.100093
  
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sciencedirect.com/science/article/pii/S266723752100148X
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Expression and purification of a cleavable recombinant fortilin from Escherichia coli for structure activity studies

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1. scibot 12 Oct 2021
  
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  RRID:Addgene_8827
  
  DOI: 10.1016/j.pep.2021.105989
  
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sciencedirect.com/science/article/pii/S1046592821001728
Aug 2021
www.mdpi.com www.mdpi.com

Development of a Broad-Spectrum Antiserum against Cobra Venoms Using Recombinant Three-Finger Toxins

1
1. scibot 16 Aug 2021
  
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  RRID:Addgene_8827
  
  DOI: 10.3390/toxins13080556
  
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mdpi.com/2072-6651/13/8/556
Apr 2021
onlinelibrary.wiley.com onlinelibrary.wiley.com

Mice deficient in the NAAG synthetase II gene Rimkla are impaired in a novel object recognition task

1
1. scibot 12 Apr 2021
  
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  RRID:Addgene_8827
  
  DOI: 10.1111/jnc.15333
  
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onlinelibrary.wiley.com/doi/10.1111/jnc.15333
Feb 2021
www.ncbi.nlm.nih.gov www.ncbi.nlm.nih.gov

Directed evolution improves the catalytic efficiency of TEV protease

1
1. scibot 25 Feb 2021
  
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  plasmid_8827
  
  DOI: 10.1038/s41592-019-0665-7
  
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ncbi.nlm.nih.gov/pmc/articles/PMC7004888/
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Functional and structural characterization of a flavoprotein monooxygenase essential for biogenesis of tryptophylquinone cofactor

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1. scibot 24 Feb 2021
  
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  RRID:Addgene_8827
  
  DOI: 10.1038/s41467-021-21200-9
  
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  Curator: @evieth
  
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nature.com/articles/s41467-021-21200-9
Jan 2021
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Purification of Affinity Tag-free Recombinant Tubulin from Insect Cells

1
1. scibot 15 Jan 2021
  
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  Addgene_8827
  
  DOI: 10.1016/j.xpro.2019.100011
  
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sciencedirect.com/science/article/pii/S2666166719300115
Sep 2020
pmc.ncbi.nlm.nih.gov pmc.ncbi.nlm.nih.gov

Mutually orthogonal nonsense-suppression systems and conjugation chemistries for precise protein labeling at up to three distinct sites

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1. scibot 16 Sep 2020
  
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  RRID:Addgene_8827
  
  DOI: 10.1021/jacs.8b12954
  
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pmc.ncbi.nlm.nih.gov/articles/PMC6500092/
onlinelibrary.wiley.com onlinelibrary.wiley.com

Wss1 homolog from Candida albicans and its role in DNA‐protein crosslink tolerance

1
1. scibot 05 Sep 2020
  
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  RRID:Addgene_8827
  
  DOI: 10.1111/mmi.14518
  
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onlinelibrary.wiley.com/doi/10.1111/mmi.14518
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G3BP1 Is a Tunable Switch that Triggers Phase Separation to Assemble Stress Granules

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1. scibot 03 Sep 2020
  
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  Addgene Cat# 8827
  
  DOI: 10.1016/j.cell.2020.03.046
  
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sciencedirect.com/science/article/pii/S0092867420303391
Jan 2020
www.nature.com www.nature.com

Cohesin cleavage by separase is enhanced by a substrate motif distinct from the cleavage site

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1. scibot 13 Jan 2020
  
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  RRID:Addgene_8827
  
  DOI: 10.1038/s41467-019-13209-y
  
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nature.com/articles/s41467-019-13209-y