Really nice transcription factor (TF) DNA-motif enrichment assays, and interesting to observe similar DNA-binding motifs for SiROCX (and Roc1), previously observed for the Aspergillus AmyR TF (doi: 10.1271/bbb.110949) and the Pf2 TF-lineage in Ascomycetes (doi: 10.1371/journal.ppat.1012536). Different lifestyles and fungal taxa, but both also closely associated with CAZyme regulation. Given the massive expansion/diversification of the Zn2Cys6 TF family in fungi it can be difficult to trace homology. Since they share similar DNA targets, it could still be interesting to include the AmyR/Pf2 Ascomycete TF lineages in an alignment/phylogenetic tree, or a domain comparison to see if you might identify any key conserved/divergent features vs the Basidiomycete ROCX TFs.
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