The way I see it, Bottom is slightly dumb for insinuating both of these things. He wishes credit for the incredible role of Pyramus, yet wishes not for the women to weep at his death. By all details of Bottom's character, he should want the ladies to cry over his demise knowing that he is Bottom.

While Bottom is shown to be the most annoying out of all the actors, the other actors are shown to be just as incompetent, if not even more in scenes like this.

While this could be her expression of annoyance with Bottom, it could also be her lack of trust in Bottom to stay with her, resulting in her wish to restrain him in some way.

Alternatively, it could simply be a manner of bedroom preference, which could also potentially explain a stressor behind her relationship with Oberon.

I view this both as an enticement and a threat. Titania can promise a life beyond what any mortal could imagine otherwise but could also summon hell on Earth if rejected. This conflicts with her very points made to Oberon about keeping the humans' peace, indicating that her permanent state of infatuation is directly affecting her sobriety/sanity to some extent.

This line could potentially be viewed either as Titania's own infatuatedly misconstrued belief or as an unintentional insult towards Bottom's character, showing that he presents as both smarter and more attractive upon saying only somewhat flirtatious thoughts in order to backpedal on his own quips.

This line is written less like one of fear and more like one of amazement, perhaps out of the suspicion that Bottom may lose his reason like a beast and attack if they act any more rudely. This potentially juxtaposes the actors' view of the lion in their play, as a lion is apparently so scary that they have to severely lighten their interpretation, while an ass shouldn't invoke fear but does impressively.

"The line emphasizes how serious the disagreement over the changeling boy has become. It is not just affecting Oberon and Titania personally..." Titania expresses the belief that her and Oberon are causing the problems that humans face in this monologue. This line specifically could have two interpretations from my perspective. She could be expressing her guilt and genuine sense of responsibility for the trouble caused while dismissing her quarrel with Oberon almost completely. Alternatively, she could also be using the fairies' general sense of responsibility for what the humans have been facing in their stir in order to pin fault on Oberon and, resultingly, dismiss his desire for Titania's new adopted obsession as only causing trouble.

"...their conflict has become so intense that she has separated herself from him." Titania's separation from Oberon here seems caused by their recent quarrel, which then seems like an extension of Titania's knowledge of Oberon's and Titania's flirtatious and chaotic behaviors towards humans. Their dispute seems to go both ways for completely different reasons.