Closing paragraph: In the last paragraph of the application letter, you can indicate how the prospective employer can get in touch with you and when are the best times for an interview. This is the place to urge that prospective employer to contact you to arrange an interview.
The first and the last are most remembered. As someone who is currently looking for employment, I make it a point to leave all forms of contact so that I'm easily accessible to contact.
If you are writing to apply for a job, begin with something like this: "I am writing to apply for the position you currently have open...."
Did you know the first few sentences are what the reader notice and remember? straight to the point or why your contacting someone is vital to not be looked over.
Deleting the salutation line altogether or inserting "To Whom It May Concern" in its place, is not ordinarily a good solution either--it's impersonal. The best solution is to make a quick, anonymous phone call to the organization and ask for a name; or address the salutation to a department name, committee name, or a position name: "Dear Personnel Department," "Dear Recruitment Committee," "Dear Chairperson,"
I was a big user of "to whom it may concern" and found it actually is impersonal. Addressing a department or specific name is much respected and appreciated.
When you do have the names of individuals, remember to address them appropriately: Mrs., Ms., Mr., Dr., and so on. If you are not sure what is correct for an individual, try to find out how that individual signs letters
Now a days it is important to correctly address the intended party and to do proper research as to whom you are addressing. Resources such as LinkedIn, emails, or even contacting the office for that information, it matters.