a. The complementary strand of DNA is: 3'--AATTACCCTGTTCGAACACATCTC--5'
b. The mRNA sequence transcribed from the complementary DNA strand is: 5'--AAU UAC CCU GUC GAA CAC AUC UC--3'
c. Using the genetic code table, the amino acid sequence is: I. Start codon: Met II. Stop codon: Stop
The given DNA sequence is 3' CGTCCACGT 5'.
The complementary strand will be built by pairing the bases: C with G, G with C, T with A, and A with T.
So, the complementary strand is 5' GCAGGTGC 3 I'd use the DNA template strand (3' CGTCCACGT 5'). In RNA, uracil (U) replaces thymine (T).
So, the mRNA sequence will be 5' GCAGGUGCA 3'.
Answer: 5' GCAGGUGCA 3'
. Assembling the Original DNA: You start with a double-stranded DNA molecule. One strand has the sequence 5'-GCAT-3', and it's paired with its complementary strand, which is 3'-CGTA-5'. Remember, A always pairs with T, and G always pairs with C.
Separating the Strands (Helicase): This is like the job of the enzyme DNA helicase. It unwinds and separates the double-stranded DNA into two single strands.
Building Daughter Strands : Each of the original strands now serves as a template for building a new, complementary strand. This is what DNA polymerase does. It adds nucleotides to the 3' end of the new strand, following the base-pairing rules. So, for the template 5'-GCAT-3', the new strand will be 3'-CGTA-5'. And for the template 3'-CGTA-5', the new strand will be 5'-GCAT-3'. Disassembling the Model: This just refers to taking apart the physical model you built to represent the DNA. It's not a step that happens in actual DNA replication in a cell.
Final Answer: DNA replication steps: assembling original DNA, separating strands (helicase), building daughter strands (DNA polymerase), and disassembling the model.
DNA replication is how a DNA molecule makes an exact copy of itself. This is crucial for cell division, ensuring each new cell gets a complete set of genetic instructions.
The process is semi-conservative, meaning each new DNA molecule has one original strand and one newly synthesized strand. This helps reduce errors during copying.
Key steps include: 1. The DNA double helix unwinds. 2. New DNA bases (A, T, G, C) are added to each original strand. 3. Two new DNA molecules are created, each with one old and one new strand.
Several enzymes are involved, including RNA primase, DNA helicase, DNA polymerase, and DNA ligase, each playing a specific role in the process.