which may lead to decreased transmission.
Doesnt seem author proposes this for humans.
10 Matching Annotations
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pmc.ncbi.nlm.nih.gov pmc.ncbi.nlm.nih.gov
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These results demonstrate that this recombinant novel HA protein is efficacious when used as a vaccine against novel H1N1 swine influenza.
Even if this vaccine was never replicated for humans, did they ever implement it at a wider level for swine populations to prevent spillover? seems relevant to what was discussed earlier.
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This is the first report of immunization of swine with a recombinant protein produced via an alphavirus replicon expression system.
This study was done primarily on pigs, and was the first report of its kind. Was it replicated for humans? If so what was the turnaround for Humans?
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It took less than two months from the time the novel HA sequence was retrieved from GISAID database until pigs were administered the first vaccine dose
This is crazy fast to go from having a sequence to having hosts replicate a protein and prevent a virus from replicating. Definitely interesting to read about in comparison to the traditional egg methodology even if I didnt understand some of the jargon here. I would be curious to know in comparison the length of making the virus typically exactly.
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On both days 4 and 5 Groups 2-4 all exhibited lower titers than Group 1
Titers refer to virus concentration, so the virus was not detected essentially as time went on.
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verage Daily Gain
This in reference to weight, It seems that if theres weight gain during the process its seen that generally there was less viral burden.
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he vaccinated Groups 2-4 demonstrated only occasional affected airways with light cuffing.
The Proteins produced seemed to have been successful in combatting the virus; the lungs showed minimal size of influenza in the pigs. It would be interesting to know the difference between 2-4 on this given the difference in concentration. Does that Matter?
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necropsy,
Essentially an autopsy for reference.
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1.14µg
Assuming 2 had the most effectiveness if the concentration was the highest.
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Western blotting performed on protein lysate confirmed expression of the novel HA protein at all the varying HA doses (Figure 1) used in vaccine preparation for the animal study.
A Western Blot is a technique where they identify proteins. It separates proteins by size using gel electrophoresis (electric current), transferring them to a membrane providing data on how much protein presence there is. in this case theyre doing this to see if the HA is detectable.
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