add silent states

the Viterbi algorithm finds the optimalpath that maximizes the observation probability of the entiresymbol sequence

Viterbi algorithm

There exist a dynamic programming algorithm,called the forward algorithm, that can compute P{x | } inan efficient manner

Even after multiple iterations, if we are getting the same centroids for all the clusters, we can say that the algorithm is not learning any new pattern, and it is a sign to stop the training.

two major roles of the ER: the synthesis and modification of proteins and the synthesis of lipids

most extensively studied of these detoxification reactions are carried out by the cytochrome P450 family of enzymes, which catalyze a series of reactions in which water-insoluble drugs or metabolites that would otherwise accumulate to toxic levels in cell membranes are rendered sufficiently water-soluble to leave the cell and be excreted in the urine. Because the rough ER alone cannot house enough of these and other necessary enzymes, a major portion of the membrane in a hepatocyte normally consists of smooth ER

In this study, we characterize the effectsof deletion of the fission yeast RNAi components on chromo-some segregation, centromere cohesion, Swi6 localization, andtelomere function during mitosis and meiosis

we have shown that the fission yeastRNAi machinery is required for epigenetic gene silencing atcentromeres (30), and for the initiation of heterochromatinformation at the silent mating type region

This telomere bouquet configuration is conserved in mosteukaryotic organisms, and is thought to facilitate meiotic pairingand subsequent homologous recombination by aligning chromo-somes

Our analysis revealed that mutations inthe RNAi machinery render the cells hypersensitive to microtubuledestabilizing drug thiabendazole, as compared with wild-type cells(Fig. 1A), indicating that the process of chromosome segregation isnot robust in the mutant strains.

In thisstudy, we examine the effects of deletions of the fission yeast RNAimachinery on chromosome dynamics during mitosis and meiosis.We find that the RNAi machinery is required for the accuratesegregation of chromosomes. Defects in mitotic chromosome seg-regation are correlated with loss of cohesin at centromeres

The fission yeast genome contains a single gene correspondingto each of the Argonaute (ago1), Dicer (dcr1), and RNA-dependent RNA polymerase (rdp1) factors required for RNAi inother systems