In some embodiments, the non-essential gene to be removed and/or replaced from the bacteriophage is the hoc gene from a T4 E. coli bacteriophage.

crRNA strain coverage and activity [0225] Objectives: (1) assess the pathotype distribution of the 625 Escherichia coli (E. coli) genomes analyzed, (2) assess the strain coverages of clustered regularly spaced short palindromic repeats (CRISPR) machinery based on genomic query for the ftsA gene target and both Type I-E and Type I-F CRISPR-Cas systems, and (3) perform a functional assessment of lethality by the combined Type I-E and Type I-F ftsA-targeting CRISPR ribonucleic acid (crRNA) complexed with the leuO expression cassette.

22. The nucleic acid sequence of any one of claims 1-20, wherein the target bacterium is E. coli. 23. The nucleic acid sequence of claim 22, wherein the E. coli is a multidrug-resistant (MDR) strain. 24. The nucleic acid sequence of claim 22, wherein the E. coli is an extended spectrum beta- lactamase (ESBL) strain. 25. The nucleic acid sequence of claim 22, wherein the E. coli is a carbapenem-resistant strain. 26. The nucleic acid sequence of claim 22, wherein the E. coli is a non-multidrug-resistant (non-MDR) strain. 27. The nucleic acid sequence of claim 22, wherein the E. coli is a non-carbapenem-resistant strain. 28. The nucleic acid sequence of any one of claims 20-25, wherein the E. coli causes urinary tract infection. 29. The nucleic acid sequence of any one of claims 22-28, wherein the E. coli causes inflammatory bowel disease (IBD).