Morphologyof Aphotosensitivegangli-on cells as revealedbyintracellular stainin
The morphology of the ganglion is extremely influenced by the amount of voltage, that is seen in the graphs for figure 2.
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- Mar 2024
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2 min) and inverse
Its just like I had mentioned in my article questionnaire doc, the first graph is really showing that high of 500 nm and the baseline voltage of 60 nm.
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A 500 nm10 mV 15.3 log ph/s/cm210 secB> 30 - ~- 400E 30 460-- o/50020 -*- 520'N 20 ! ~ ,o570/10. /3 - 600? !13 14 15 16Log Irradiance(photons/s/cm2)C-253 -e- cell 62-4) -3 -o -A- all cellsnomogram-4400 500 600Wavelength(nm)Fig. 2. Spectral tuning of light response inphotosensitive ganglion cells. (A) Voltage re-sponses of a single cell to a 500-nm narrow-band stimulus at indicated intensities (in log1ophotons s-1 cm-2). Baseline - -60 mV foreach trace. (B) Plots of peak depolarization as afunction of log retinal irradiance for each ofseveral narrow-band spectral lights (400- to600-nm wavelength, as indicated; 10-nm widthat half height); same cell as in (A). Peak wasobtained from a 1-s boxcar average of rawvoltages. (C) Spectral sensitivity functions de-rived for photosensitive ganglion cells from rel-ative displacements of intensity-response func-tions along the abscissa in (B). Red curve: samecell as in (A) and (B). Green curve: group datafor all cells (n = 34; number of cells tested perwavelength as follows: 400 nm, 5; 420 nm, 3;440 nm, 2; 460 nm, 4; 480 nm, 4; 500 nm, 34;520 nm, 7; 540 nm, 5; 570 nm, 2; and 600 nm,2). Black curve: nomogram for retinal -basedphotopigment with kmaxof 484 nm (411, fit byleast squares method to the group data.www.sciencemag.org SCIENCE VOL295 8 FEBRUARY2002 1071This content downloaded from 137.22.14.85 on Fri, 17 Apr 2015 19:40:35 UTCAll use subject to JSTOR Terms and Conditions
These graphs are very descriptive. Having the highs and lows of. the voltage stimulation is very good to see.
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Plots of peak depolarization as afunction of log retinal irradiance for each ofseveral narrow-band spectral lights
.The graph illustrates many points of interest. The amount of voltage being emitted is showing how the peak depolarizations differ.
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Voltage re-sponses of a single cell to a 500-nm narrow-band stimulus at indicated intensities (in log1o
According to the graph the sensitivities hit peaks and valleys significantly throughout the test. That shows the inconsistencies with this measuring tactic
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Detachment from pigment epitheliumand exposure to bright light duringdissection
How would this have a significant effect?
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Judgingfrom available spectral evidence, the pho-topigment in these ganglion cells is morelikely to be a retinaldehyde-based opsinsuch as melanopsin (5, 19, 20)
I would be curious to see a side-by-side comparison of how these are similarly related.
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which blocks calcium-mediatedsynapticrelease from rods, cones, and other retinalneurons
This is a great thing to mention. The blocking of calcium is very nice to have noted for how the reaction occurs.
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