- Jun 2017
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sites.google.com sites.google.com
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receptor-mediated endocytosis begins
Both West Nile Virus and Norovirus enter the cell through receptor-mediated endocytosis
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positive-sense single stranded RNA
Both West Nile Virus and Norovirus have a genome structured as positive-sense single-stranded RNA
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sites.google.com sites.google.com
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Internalized
Both West Nile Virus and Norovirus enter the cell through receptor-mediated endocytosis
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Positive-sense RNA strand ((+)RNA)
Both West Nile Virus and Norovirus have a genome structured as positive-sense single-stranded RNA
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- May 2017
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Microbiology of Community-Acquired Acute Bacterial Rhinosinusitis
numbers
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ic.ucsc.edu ic.ucsc.edu
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These proteases play a prominent role inthe pathogenesis of experimental pneumonia(Lyerly and Kreger, 1983) and keratitis (Lyerlyet al., 1981).
virulence
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www.ncbi.nlm.nih.gov www.ncbi.nlm.nih.gov
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Serratia marcescens was the most common organism isolated from the corneal scrapings and from the contact lenses.
Common
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www.microbiologyresearch.org www.microbiologyresearch.org
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Pathogenicity and virulence
Virulence factors
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www.ncbi.nlm.nih.gov www.ncbi.nlm.nih.gov
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[Drug sensitivity of Yersinia enterocolitica and Yersinia pseudotuberculosis].
one study of antibiotic resistance
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www.cdc.gov www.cdc.gov
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TABLE 1. Number of cases and incidence of confirmed infections,* hospitalizations, and deaths, by pathogen — Foodborne Diseases Active Surveillance Network, United States, 2015†
incidence
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www.ncbi.nlm.nih.gov www.ncbi.nlm.nih.gov
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As control measures, the distributor improved hygiene measures, and the military bases thoroughly cleaned the bathrooms and kitchens, and increased awareness on hand hygiene among the soldiers. From Week 21, the kitchens in all three bases in Troms voluntarily elected to close the salad bar and refrain from serving pork products until the outbreak was resolved.
public health interventions
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www.ncbi.nlm.nih.gov www.ncbi.nlm.nih.gov
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National outbreak of Yersinia enterocolitica infections in military and civilian populations associated with consumption of mixed salad, Norway, 2014.
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en.wikipedia.org en.wikipedia.org
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They have no specific growth requirements and grow well on standard laboratory media, but grow best between 35 and 37 °C and at pH 7.2.
Growth requirements for Klebsiella genus
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www.cdc.gov www.cdc.gov
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A total of 5,093 isolates were collected in 2014. Of these, 25.3% were resistant to tetracycline, 19.2% to ciprofloxacin, and 16.2% to penicillin (plasmid-based, chromosomal, or both)
additional antibiotic resistance numbers from one study by cdc
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n 2010, 27.2% of all GISP isolates were resistant to penicillin, tetracycline, ciprofloxacin, or some combination of those antimicrobials and 6.9% of isolates were resistant to all three antimicrobial
antibiotic resistance numbers
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www.ncbi.nlm.nih.gov www.ncbi.nlm.nih.gov
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62 million new cases of gonorrhea occur globally each year
prevalence
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www.aafp.org www.aafp.org
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Disseminated infection is rare but can occur 1 to 3 percent of adults
Disseminated infection
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ceftriaxone in a single 125-mg dose intramuscularly or ciprofloxacin (Cipro) in a single 500-mg dose orally,
antibiotic treatment
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usually do not cause adverse sequelae
Sequelae
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microbewiki.kenyon.edu microbewiki.kenyon.edu
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Virulence Factor
virulence factors
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www.ncbi.nlm.nih.gov www.ncbi.nlm.nih.gov
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OUTER MEMBRANE CONSTITUENTS ASSOCIATED WITH GONOCOCCAL VIRULENCE
attachment
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www.aafp.org www.aafp.org
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Up to 90 percent of pharyngeal gonorrhea in men and women may be asymptomatic
numbers for asymptomatic infection
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URL
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www.ncbi.nlm.nih.gov www.ncbi.nlm.nih.gov
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The oxidase test uses the tetramethyl derivative of the oxidase reagent
oxidase test
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The catalase test (3% hydrogen peroxide) or superoxol (30% hydrogen peroxide) are other rapid tests used in the presumptive identification of N gonorrhoeae. A drop of the reagent is placed in the centre of a clean glass slide and the suspect colony is picked with a loop and emulsified in the reagent. N gonorrhoeae will produce a positive reaction with bubbling within 1 s to 2 s. Weak bubbling or bubbling after 3 s indicates a negative reaction (5) (Table (Table2).2).
Catalase test
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The inoculated plates should be incubated at 35°C to 37°C in a moist atmosphere enriched with CO2 (3% to 7%) (5). An 18 h to 24 h culture should be used as the inoculum for additional tests. Plates should not be incubated for longer than 48 h because most old cultures would not survive storage conditions.
Culture conditions
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intracellular Gram-negative kidney-shaped diplococci in polymorphonuclear leukocytes
Gram stain morphology
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www.nytimes.com www.nytimes.com
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Symptoms
Symptoms
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www.ncbi.nlm.nih.gov www.ncbi.nlm.nih.gov
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More than 90% of staphylococcal isolates now produce penicillinase
Penicillin resistance of staph
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- Apr 2017
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www.ncbi.nlm.nih.gov www.ncbi.nlm.nih.gov
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The overall mortality rate was 15%, with no significant difference between groups.
MRSA mortality rate
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journal.frontiersin.org journal.frontiersin.org
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open – labeled studies
A study in which both the researchers and the patient knows which drug the patient is given.
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bacteriostatic
Stops bacteria from reproducing, but does not necessarily kill them.
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vancomycin exposure after colonization as the strongest predictor of prolonged colonization
Allowed to grow better when there is less competition from vancomycin-susceptible bacteria?
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only a handful
There are 3 different kinds of Vancomycin-resistant S. aureus that vary in how resistant they are.
https://en.wikipedia.org/wiki/Vancomycin-resistant_Staphylococcus_aureus
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Neutropenia
Fewer neutrophils in the blood
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APACHE scores
Indicates severity of disease
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q6h
Every 6 hours
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www.nejm.org www.nejm.org
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Our effort to identify known virulence genes revealed a surprising deficiency of toxin genes other than pvl, lukD, and lukE.
virulence genes
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www.ncbi.nlm.nih.gov www.ncbi.nlm.nih.gov
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These events cause the symptoms of septic shock that occur during severe infections caused by S aureus.
Toxicity
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S aureus cells express on their surface proteins that promote attachment to host proteins such as laminin and fibronectin that form part of the extracellular matrix
Attachment
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The catalase test is important in distinguishing streptococci (catalase-negative) staphylococci which are catalase positive.
Distinguish between staph/strep
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wcms-wp.cdc.gov wcms-wp.cdc.gov
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more likely to occur in children younger than 5 years and people with weakened immune systems.
Does the friend have a weakened immune system?
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Later, the person's lymph nodes closest to the original scratch or bite can become swollen, tender, or painful.
Do they have swollen areas/bumps, especially near a bite/scratch?
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About three to 14 days after the skin is broken, a mild infection can occur at the site of the scratch or bite.
Did they recently have a skin infection where they may have gotten bitten/scratched?
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Cat-scratch disease (CSD) is a bacterial infection spread by cats.
Have they been in physical contact (bitten, licked, etc.) with a cat?
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www.cnn.com www.cnn.com
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pigs are 'mixing vessels,'
Pigs are susceptible to both avian and human flu viruses, making them especially effective mixing vessels
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In less than four months, about 4,000 cases and 550 deaths from SARS could be traced to Liu's stay in Hong Kong.
The CDC states that in the 2003 outbreak, there were a total of 8,098 cases of SARS, with 774 deaths. 8 of these cases were in the United States.
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no longer needs the animal reservoir
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