However, we found that suppressing yellow expression in the larval CNS, dopaminergic neurons, or serotonergic neurons (Figure 2—figure supplement 3, FET, P values ranging from 0.45 to 1), or in all neurons (Figure 2E, FET, p=1 in all cases) or all glia (Figure 2F, FET, p=1), had no significant effect on male mating success.
This result was surprising because it shows that turning off yellow in key parts of the nervous system—including neurons involved in mood and movement—does not affect male mating behavior. This suggests that yellow doesn’t act in the brain or nerves to influence mating, challenging the original hypothesis.