surveillance in wastewater, an initiative advocated at European andglobal level, and recommended by the European Commission and theWorld Health Organization (
Have any countries adopted this methodology for epidemiology?
9 Matching Annotations
- Feb 2026
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Differently, the S gene was detected, with both as-says, only in the water samples collected on January 11th and February1st 2021, while in December 2020, it was observed only in samples an-alyzed using the single assay.
Is this because the S gene allows for mutations/variants?
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In addition, multiplex is faster since itreduces by three fold the number of replicates, assuming that for eachtarget gene the analysis of three replicates is performed.
This ties back into my previous annotation. It's great that this approach is so fast but at what point does troubleshooting make multiplexing less efficient than singleplexing?
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During the setup of the multiplex, we observedcompetition for reagents, reason for which one of the three genescould not amplify.
At what point do complications with multiplex PCR, such as this one, make this approach less worthwhile than a singleplex PCR?
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good laboratory practices wereobserved i.e. using different laboratory locations for sample processing,RNA extraction, reverse-transcription of samples and qPCR.
This is a SUPER important practice within this study and in general. Contamination, especially in forensics, can produce devastating results within the criminal justice field and your own credibility. It is great that they included this, even if the study isn't based in forensic science.
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A total of four untreated wastewater samples were collected fromthe influent of a WWTP located in Lombardy area (North Italy) betweenDecember 2020 and February 2021.
How would this experiments results change depending on the region where the wastewater was recovered?
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Some multiplex protocols were developed for a dual detection ofSARS-CoV-2 and influenza virus
When I visited urgent care last December, they did a multiplex on me to test for COVID, strep, and influenza. Was this technology being developed before the COVID outbreaks, or was it truly born out of necessity?
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ndeed, in low-income countries, WWTP monitoring can be an important tool for thefight against COVID-19 spread
how many countries/states/etc. are actually doing this for RNA viruses?
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shows low stabilityin the environment (La Rosa et al., 2020a), the presence of viral geneticmaterial in the stool of positive subjects could reflect the abundance ofSARS-CoV-2 RNA content in untreated wastewaters
how did they discover this fact in the first place?
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