under general apoptotic stress, Tip30 expression significantly improved yeast resistance to acetic acid–induced growth inhibition
This is very cool! Is this experiment with wild-type or fmp52∆ cells?
9 Matching Annotations
- Dec 2025
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Endogenous GFP tagging did not yield clear results likely due to low protein expression
It looks like there's at least one putative UPRE upstream of FMP52 (425 bases upstream of the ATG, 5'-TACGTGT-3')! I'm curious if you tried looking at endogenous Fmp52-GFP with t-2-hex or other ER stress-inducing treatment, as it may get upregulated and be more visible than in unstressed conditions? This would be consistent with your pFMP52-luciferase results.
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- Nov 2025
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Continued efforts on engineering LNP formulations and saRNA designs will further improve transfection efficiency, cell type specific targeting, and brain specific targeting.
It will be interesting to see how organismal age and disease state impact hm5C saRNA stability/efficacy!
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robust mCherry fluorescence was present in some neurons
How does the number of saRNA-positive cells compare to the time points shown in Figure 2? It would be interesting to have micrographs from earlier time points at the same magnification of those in Supp. Fig. 1. I'm also curious if there's something measurably different about the cells still expressing saRNA after three months or if it's just a random decrease in cell cout over time.
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Surprisingly, mCherry expression was also evident throughout the corpus callosum, suggesting robust labeling of neuronal axons or myelin.
These expression patterns between treatments and across time are so striking! How much does this vary between individuals? For example, the patterns between mRNA and saRNA on day 14 are very distinct from one another and I'm curious if these types of differences are consistent.
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- Oct 2025
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Of the 39 unique key RBPs (the union of the top 20 motif-enriched RBPs from each tissue), only five (ESRP1, FUS, MBNL1, PCBP2, RBM8A) overlapped with a previously curated list of genes whose expression correlates with MLS9.
It could be useful to also assess proteomic data for these RBPs, as RNA abundance often doesn’t correlate with protein abundance, especially in the context of aging.
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although alternative first exon (AF) was the second most frequent AS event, averaging 22.8% in a single species, comparable to cassette exon (Fig. 1b), none of these were conserved
Since maximum lifespan is not a conserved phenotype, it would be interesting to see if other types of splicing events (e.g. alternative first exons) are enriched for specific biological functions, irrespective of their conservation.
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- Sep 2025
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our data raise the possibility that TBC1D15 localized to mitochondria in neurons treated with LLOMe
Is it possible that under these conditions, neurons transfer damaged mitochondria and/or other cellular material to astrocytes? It could be interesting to do neuron-specific labeling prior to co-culturing to track mitochondria.
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Thus, the lack of robust ESCRT recruitment is not simply attributed to insufficient protein expression in neurons.
I’m curious if you looked at CHMP2B levels by western blot in addition to immunofluorescence. Is it possible that neurons express alternate mRNA isoforms and/or proteoforms (e.g. truncated CHMP2B) that disrupt canonical ESCRT recruitment?
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