This manuscript has a video abstract available - made by the authors. You can watch the video in YouTube here: https://www.youtube.com/watch?v=3En7OyAwv_U

More information on the usefulness of this rich dataset is available in GigaBlog - http://gigasciencejournal.com/blog/penguin-genome/

Taonga - Maori word for "treasure" - an object or natural resouce that is highly prized.

Not talking about CRISPR - trained as plant biologist. Plants have to adapt to environments using unique strategies through chemistry. Analysing 1000s of plant tisses using HT mass spec. Plants natural products in everyday life - spices, coffee etc. and also used in mainstream medicine.

Focusing on the evolutionary assembly of specialized metabolic pathways - going to highlight how to tackle vastly unknown specialized metabolism.

Utilizing multi-omics approach - developing workflow to study very specialized traits in non-model eukaryotes. Doing fine sampling - roots, stems, flowers, etc. --> RNA-seq and high-resolution metabolomics. Then using bioinformatics to correlate molecular of interest with genes in transcriptomic database. Then clone genes.

Then - transplant the whole pathway into a different ecosystem to obtain high value natural products.

Polynesia - Kava kava plant (related to black pepper). Special plant to polynesia. Prepare tea from plant root . Why is this a special plant? Has Kacalactones, flavokaans and Alkaloids .

Bio-luminescence and systems in nature - stable isotope assisted metabolomics (SIAM) as a tool for deciphering luciferin biosynthesis.

Crowd funding - firefly genome project.

Jun Wu (Salk Institute) - Topic on Genome Editing - Homology Independent targeted Integration. Is a stem cell biologist. Problem - loss of function mutations - treatment strategy is by gene therapy. Most common is viral-mediated gene replacement but lots of issues with this e.g. copy number, insertional mutagenesis and immune reactions. What is better is site-specific transgene integration (HDR) - homology directed repair. But has low efficiency and is not applicable to non-dividing cells.

NHEJ (G0, G1, S, G2, M phases) vs. HDR (mostly active in S-G2 cell cycle phase).

HITI - introduces a donor plasmid with a guide RNA sequence. Inserted in reverse direction.

LUO - also interested in epigenome editing - targeted DNA methylation by dCAS9 methyltransfereases (CRISPRme) - WG bisulfite sequencing reveals novel off-target characteristics of CRISPRme.

LUO using CRISPR genome editing for regenerative research/ applications. Has shown that chromatin structure can affect genome editing activities. Gene editing activity of CRISPR-Cas9 correlated with secondary structure of gRNA guide sequences.

Data type: workflow

People may want to add data type: VM as well. Or add a new category: Containers

Data type: workflow

People may want to add data type: VM as well. Or add a new category: Containers

DataType: workflow