Uncovering secrets of the proteome: Alternate RNA decoding & Protein asymmetry shaping cell fate

This presentation "Uncovering the secrets of the human proteome" was given in May 2024 at the 50th anniversary celebration of the Barnett Institute. It focused on progress in proteomics and two new discoveries: 1. Alternate RNA decoding results in stable and abundant proteins in mammals 2. Proteome asymmetry in mouse and human embryos before fate specification

The vastness and complexity of the human proteome have hampered its exploration. New mass spectrometry technologies are transcending those limitations and allowing for large gains in sensitivity, sequence coverage, spatial and temporal resolution. I will discuss the conceptual drivers of this progress and provide examples of how it will advance our understanding of the human proteome and enable better therapeutics.

https://youtu.be/F4-PUuz5kcQ?si=5iHIshGprYutcRih

Primer and trends in single-cell mass spectrometry proteomics l Prof. Nikolai Slavov l SCP2024

https://youtu.be/kGNhq_8u4pE?si=F0P-hnLpVGWVsHVK

Primer and trends in single-cell mass spectrometry proteomics l Prof. Nikolai Slavov l SCP2024

https://youtu.be/kGNhq_8u4pE?si=F0P-hnLpVGWVsHVK

This presentation "From proteome variation across single cells to molecular mechanisms" was given in Nov 2024 at the EMBL conference "Quantitative biology to molecular mechanisms". It focused on the progress of proteomics in two directions: 1. Alternate RNA decoding results in stable and abundant proteins in mammals 2. The potential of (sub-)single-cell proteomics to infer functional regulation

https://www.youtube.com/watch?v=LN4UWvnlmng

Single-nucleus proteomics identifies regulators of protein transport l Dr. Jason Derks l SCP2024

https://youtu.be/soE9z1ECiHs?si=_gcpArv7CiweTOVn

Systematic inference of post-transcriptional regulation l Saad Khan & Megan Elcheikhali l SCP2024

https://youtu.be/jyh9Z9JgEzs?si=GqqNwgpNUMuWsbvG

This presentation "From proteome variation across single cells to molecular mechanisms" was given in Nov 2024 at the EMBL conference "Quantitative biology to molecular mechanisms". It focused on progress of proteomics in two directions: 1. Alternate RNA decoding results in stable and abundant proteins in mammals 2. The potential of (sub-)single-cell proteomics to infer functional regulation

https://www.youtube.com/watch?v=LN4UWvnlmng

Exploring functional protein covariation across single cells | Prof. Nikolai Slavov | SCP2022

https://youtu.be/GWHzvHGvUTg

Exploring functional protein covariation across single cells | Prof. Nikolai Slavov | SCP2022

https://youtu.be/GWHzvHGvUTg

Prioritized single-cell proteomics | Gray Huffman | SCP2021

https://youtu.be/SP0x3gAALtg

Prioritized single-cell proteomics | Gray Huffman | SCP2021

https://youtu.be/SP0x3gAALtg

Using DIA-NN to analyze plexDIA data | Jason Derks

https://youtu.be/0Wmg9LjDtgE

Progress, challenges and standards for single cell proteomics | Nikolai Slavov | SCP2018 https://youtu.be/tmL4i5SyE2s

Single Cell Proteomics by Mass-spec | CSHL Meeting: Single Cell Analyses 2017 https://youtu.be/D4JtnM-4Lds

A presentation for ASMS2021 | Increasing the throughput of sensitive proteomics by plexDIA | Prof. Nikolai Slavov https://youtu.be/LDd5B_x4JPE

Droplet sample preparation for single-cell proteomics applied to the cell cycle. This presentation was recorded for keynote talks at the 10th AOHUPO and e-MSB 2021. https://youtu.be/chinY96ngi0

Scaling up single-cell analysis to the proteome | CZI Webinar https://youtu.be/ezWu0FPwwTk

Droplet sample preparation for single-cell proteomics | Andrew Leduc | SCP202 https://youtu.be/DJ1U_KpMNcY

High-throughput single-cell proteomics quantifies the emergence of macrophage heterogeneity | SCP2019 https://youtu.be/NNLh4nE687I

Optimizing single-cell analysis of proteins and RNAs | Nikolai Slavov | SCP2020 https://youtu.be/LkNMskjxYXA