Line 45: Also, like toxin/antitoxin systems (the most dominant phage inhibition systems) combining with type IV restriction system McrBC to thwart T2 infection (see https://journals.asm.org/doi/10.1128/spectrum.03388-23 , ).
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www.biorxiv.org www.biorxiv.org
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We previously studied resuscitation with all 20 aa’s (2020) and found Ala increases persister resuscitation (https://doi.org/10.1016/j.isci.2019.100792 ) in a manner different from the other 19 aa's, so this should be referenced.
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Also, ours is the only mechanistic model for persister cell formation and resuscitation and should be cited as well (2020, https://doi.org/10.1016/j.bbrc.2020.01.102 ), as we have already “elucidates the regulatory mechanism of metabolic pathways” related to persistence and resuscitation.
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Moreover, we were the first to show reducing ATP increases persistence (2013, doi:10.1128/AAC.02135-12), not your ref 21 & 22 (2024 & 2016).
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P 6: This statement is patently false: “it is not clear which metabolic pathways during dormancy control resuscitation in Bacteria” as we have found the only mechanism (https://doi.org/10.1016/j.isci.2019.100792) from ppGpp to ribosome inactivation leading to persistence to reduction of ppGpp and activation of ribosomes with HflX.
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P 6/7: we discovered the impact of Cm and ATP levels and dormancy (doi:10.1128/AAC.02135-12).
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page 10: More conceptional plagiarism: "For instance, amino acids act as signals for latent spores in Gram positive bacteria (26). In addition, our data indicate that several amino acids involved in central carbon metabolism are altered in rapidly regrowing dormant bacteria (Fig. 1). Thus, we hypothesized that these metabolites could serve as signals or essential nutrients for rapid bacterial resuscitation from dormancy." This hypothesis is not only already published, it was tested and shown to be correct in 2020 (https://doi.org/10.1016/j.isci.2019.100792 ).
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There are no 'fast-growing' and 'slow-growing' persisters as several labs have shown that when cells resuscitate, they all grow remarkably at the same rate as shown first in 2018 (https://enviromicro-journals.onlinelibrary.wiley.com/doi/10.1111/1462-2920.14093).
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p 10: more conceptional plagiarism: D-Ala was used previously to test resuscitation with same strain (https://doi.org/10.1016/j.isci.2019.100792).
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p 14: The ribosome requirement for resuscitation is not "likely" as it is proven (https://enviromicro-journals.onlinelibrary.wiley.com/doi/10.1111/1462-2920.14093).
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