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    A Hybrid Adherent/Suspension Cell-Based Selection Strategy for Discovery of Antibodies Targeting Membrane Proteins
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    1. wowsee 07 Apr 2026
      The next day, passage yeast to an OD of 1 in 1 L fresh SDCAA. Incubate the culture overnight at 30 °C while shaking at 200 rpm.

      Two-stage growth protocol... does this do anything to ensure that inoculation of induction media is timed for when yeast are in log phase?

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    link.springer.com/protocol/10.1007/978-1-0716-2285-8_11