Transfection of not cell cultures, but whole organisms is now a possible feat. Often this is done using a virus-based transfection system.
The virus contains the gene you want expressed in the organism and that virus then goes and infects cells. These viruses can be specifically designed to target certain organs like the liver or brain, so that only the organ you want has that DNA and is expressing that protein.
This process is becoming so safe and efficient that it is now being used in extreme circumstances on humans. If a person suffers from a disease caused by a mutation in a gene, a working version of it could theoretically be inserted into their cells using viruses.
Often this concept is lumped together under the broad term "genetic engineering," but is more appropriately termed "gene therapy."
Although this field has been plagued with many past disasters, new advances and better understanding of viral biology is making this a viable treatment for many diseases. The field of gene therapy is still recovering after the death of Jesse Gelsinger, among others.