2 Matching Annotations
  1. Sep 2021
  2. Local file Local file
    NT-MO-029 AppNote.indd
    2
    1. zhentg 17 Sep 2021
      because of its small size, binding of tris-NTA has minimum effect on biochemical and physicochemical properties of the protein

      small tag may not alter the conformation and function of the protein

      his tag
    2. zhentg 17 Sep 2021
      The observed Kd values were 1.3 ± 0.2 nM for the His6 peptide, 0.6 ± 0.3 nM for IDH R132H and 2.4 ± 1.1 nM for p38α

      2 points:

      1. binding Kd is about 1nM
      2. Kd for different targets could be slightly different
      MST his tag

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