3 Matching Annotations
  1. May 2019
    1. high osmolarity conditions (Gowrishankar, 1989; Csonka, 1989) for β-galactosidase assay
    2. Assays for determination of β-galactosidase enzyme activity in cultures were performed as described by Miller (1992) after permeabilizing the cells with SDS/chloroform, and the activity values were calculated in Miller units, as defined therein. For determination of proU activity from a proU::lac fusion that contains the proUpromoter cloned upstream of the lacZYA genes (as in plasmid pHYD272), cultures used were grown in LBON or K-medium (low osmolarity medium) since proU is also induced under
    3. β-Galactosidase assay