17 Matching Annotations
  1. May 2019
    1. Fixative : 4% Formaldehyde
    2. Ethylene Glycol Tetraacetic acid (EGTA), pH 7.0ComponentsFinal concentrationFor 50 mlEGTA0.1M1.902gH2Oq.sThe pH is adjusted to 7.0 using 10M NaOH
    1. Extraction buffer
    2. 10XBinding buffer
    3. Agarose gel
    4. Nuclear lysis buffer (without protease inhibitors
    5. Permeabilisation buffer: 0.2% Triton X100
    6. Stripping buffer
    7. Blocking buffer
    8. TBS-T
    9. Transfer buffer
    10. (f) Running buffer
    11. (e) Stacking polyacrylamide gel
    12. (d) Resolvingpolyacrylamide gel
    13. (c) 6X Protein loading buffer (Lammeli buffer)
    14. (b) Celllysis buffer B(For IB)
    15. Cell lysis bufferA(For IP)