3 Matching Annotations
  1. Jan 2020
    1. The fundamental issue surrounding bacterial culture growth measurements is that an absorbance spectrophotometer is being asked to determine light scattering caused by particulates in suspension. In this case, transmittance is not related to absorbance in the classical sense. Under normal true absorbance conditions, spectrophotometers can be comparable to one another because the sample actually absorbs electromagnetic energy. In the case of a reduction of transmittance caused by light scattering, readings are very dependent on the optics of a specific spectrophotometer as well as the cell type in suspension
  2. Dec 2019
    1. we recommend calibrating OD using serial dilution of silica microspheres, which readily produces highly precise calibration (95.5% of teams having residuals less than 1.2-fold), is easily assessed for quality control, and as a side effect also assesses the effective linear range of an instrument.
  3. Aug 2016
    1. to America or the Colonies

      As Steve Jones says in my attached article, the 19th century relationship between the US and Britain was actually quite strong. Doyle is using this brief mention of America to display the relationship between the nations at the time. Though America became independent from the UK in 1776, by the 1800's it has become quite reasonable for someone to possibly seek refuge in "the Colonies".