On 2014 Apr 07, Muhammad Aslam commented:

This is an interesting study but there is a technical mistake. Hopefully the authors can respond to that. On page 1351 (page 2 of article) the authors state ''Here, we have used 2 such Ras effector mutants to identify selective contributions of Ras effectors of the extracellular signal-regulated kinase (ERK)/mitogen-activated protein kinase (MAPK) or PI3K pathway to vascular phenotypes in vivo. RasV12C40 (G12→V12, T40→C40) binds to and selectively activates PI3K, whereas RasV12S35 (G12→V12, Y35→S35) binds to Raf1 and selectively activates the ERK/MAPK pathway.''

In original H-Ras sequence (NP_001123914.1) there exist no T40 or Y35 rather it is the other way round. In sequence it is T35 and Y40 which is changed to S35 and C40, respectively, as stated by article from Joneson T et al (Science 1996; 271: 810–812).

Regards,

On 2014 Apr 07, Muhammad Aslam commented:

This is an interesting study but there is a technical mistake. Hopefully the authors can respond to that. On page 1351 (page 2 of article) the authors state ''Here, we have used 2 such Ras effector mutants to identify selective contributions of Ras effectors of the extracellular signal-regulated kinase (ERK)/mitogen-activated protein kinase (MAPK) or PI3K pathway to vascular phenotypes in vivo. RasV12C40 (G12→V12, T40→C40) binds to and selectively activates PI3K, whereas RasV12S35 (G12→V12, Y35→S35) binds to Raf1 and selectively activates the ERK/MAPK pathway.''

In original H-Ras sequence (NP_001123914.1) there exist no T40 or Y35 rather it is the other way round. In sequence it is T35 and Y40 which is changed to S35 and C40, respectively, as stated by article from Joneson T et al (Science 1996; 271: 810–812).

Regards,