On 2017 Dec 06, Alexander Kraev commented:

The authors of this paper claim to have generated mouse strains that carry a PLN(R9C) transgene and 2, 1, or 0 endogenous PLN alleles. However, the mutant PLN resides on a transgenic array, with multiple copies the number of which the authors chose not to determine. It was independently found (https://doi.org/10.1101/075671), that the mutant PLN gene, assuming the transgene does not re-arrange during breeding, is present in 13 copies. This work also did not determine the actual mutant to wildtype PLN ratio at the mouse age, when the expression of the transgenic promoter is maximal (1 month), which would have estimated the scope of the overexpressed protein "bolus injection" that each strain received. Therefore, the conclusions of this paper should be interpreted with extreme caution, especially as such gene combinations never occur in human patients carrying PLN mutations.

On 2017 Dec 06, Alexander Kraev commented:

The authors of this paper claim to have generated mouse strains that carry a PLN(R9C) transgene and 2, 1, or 0 endogenous PLN alleles. However, the mutant PLN resides on a transgenic array, with multiple copies the number of which the authors chose not to determine. It was independently found (https://doi.org/10.1101/075671), that the mutant PLN gene, assuming the transgene does not re-arrange during breeding, is present in 13 copies. This work also did not determine the actual mutant to wildtype PLN ratio at the mouse age, when the expression of the transgenic promoter is maximal (1 month), which would have estimated the scope of the overexpressed protein "bolus injection" that each strain received. Therefore, the conclusions of this paper should be interpreted with extreme caution, especially as such gene combinations never occur in human patients carrying PLN mutations.