On 2014 Jul 04, Ferenc Zsila commented:

In page 583, the authors claim that "We also examined anthocyanidin–DNA interactions spectrophotometrically at pH 7.5 but were unable to demonstrate significant or consistent shifts in l max or maximum absorbance that would indicate the interaction between the two at this pH as has been observed for other flavonoids (Webb and Ebeler 2004)."

However, all details of the spectrophotometric measurements are missing from the experimental section. Name and concentration of the tested anthocyanidin(s), concentration of the DNA, temperature, solvent, optical pathlength, wavelength range, and the type of the instrument used all remain unknown. Additionally, the absorption spectra are not shown either.

On 2014 Jul 04, Ferenc Zsila commented:

In page 583, the authors claim that "We also examined anthocyanidin–DNA interactions spectrophotometrically at pH 7.5 but were unable to demonstrate significant or consistent shifts in l max or maximum absorbance that would indicate the interaction between the two at this pH as has been observed for other flavonoids (Webb and Ebeler 2004)."

However, all details of the spectrophotometric measurements are missing from the experimental section. Name and concentration of the tested anthocyanidin(s), concentration of the DNA, temperature, solvent, optical pathlength, wavelength range, and the type of the instrument used all remain unknown. Additionally, the absorption spectra are not shown either.