- Jul 2018
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europepmc.org europepmc.org
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On 2014 Oct 15, Amanda Capes-Davis commented:
More recent work has shown that hTERT-EEC is misidentified and is actually MCF-7. So hTERT-EEC cells are from breast carcinoma, not immortalized endothelium.
It's important to test cell lines to confirm they correspond to the expected donor and are not cross-contaminated, using a consensus technique such as STR profiling. Journals are increasingly requiring testing as a prerequisite before publication and this will help to address the widespread use of misidentified cell lines in the scientific literature.
It's also a good idea to check before using a cell line to see if others have documented a problem previously. ICLAC maintains a list of known misidentified cell lines at http://iclac.org/databases/cross-contaminations/.
This comment, imported by Hypothesis from PubMed Commons, is licensed under CC BY.
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- Feb 2018
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europepmc.org europepmc.org
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On 2014 Oct 15, Amanda Capes-Davis commented:
More recent work has shown that hTERT-EEC is misidentified and is actually MCF-7. So hTERT-EEC cells are from breast carcinoma, not immortalized endothelium.
It's important to test cell lines to confirm they correspond to the expected donor and are not cross-contaminated, using a consensus technique such as STR profiling. Journals are increasingly requiring testing as a prerequisite before publication and this will help to address the widespread use of misidentified cell lines in the scientific literature.
It's also a good idea to check before using a cell line to see if others have documented a problem previously. ICLAC maintains a list of known misidentified cell lines at http://iclac.org/databases/cross-contaminations/.
This comment, imported by Hypothesis from PubMed Commons, is licensed under CC BY.
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