On 2015 Mar 13, Martine Crasnier-Mednansky commented:

Escherichia coli cells growing on excess glucose (Fig. 3, Environment G) do not consume acetate after glucose depletion (the acetate switch occurs before glucose is fully depleted). In fact, cells utilize both glucose and acetate during entry into the stationary phase of growth (see Fig. 1A in Wolfe AJ, 2005). Failure to assimilate acetate before glucose depletion was reported for a specific mutant strain and resulted in a diauxic type of growth (Nyström T, 1993).

Acetate utilization by acetate-adapted cells is not diminished by glucose addition (Lowry OH, 1971). Furthermore glucose utilization by acetate-adapted cells is inhibited by acetate. It is therefore questionable whether acetate-adapted cells are `adapting´ to glucose when transferred to a medium containing a large excess of acetate (Fig. 3, Environment GA). In this context, the reversible phosphotransfer reaction between phosphoacetate kinase and the phosphotransferase system (PTS), originally proposed by Fox DK, 1986 but never established in vivo, should be physiologically relevant as to regulate the rate of sugar transport by the PTS and thus the cAMP level.

On 2015 Mar 13, Martine Crasnier-Mednansky commented:

Escherichia coli cells growing on excess glucose (Fig. 3, Environment G) do not consume acetate after glucose depletion (the acetate switch occurs before glucose is fully depleted). In fact, cells utilize both glucose and acetate during entry into the stationary phase of growth (see Fig. 1A in Wolfe AJ, 2005). Failure to assimilate acetate before glucose depletion was reported for a specific mutant strain and resulted in a diauxic type of growth (Nyström T, 1993).

Acetate utilization by acetate-adapted cells is not diminished by glucose addition (Lowry OH, 1971). Furthermore glucose utilization by acetate-adapted cells is inhibited by acetate. It is therefore questionable whether acetate-adapted cells are `adapting´ to glucose when transferred to a medium containing a large excess of acetate (Fig. 3, Environment GA). In this context, the reversible phosphotransfer reaction between phosphoacetate kinase and the phosphotransferase system (PTS), originally proposed by Fox DK, 1986 but never established in vivo, should be physiologically relevant as to regulate the rate of sugar transport by the PTS and thus the cAMP level.