On 2015 Nov 17, Prof.Dr.Jogenananda Pramanik commented:

Thyroxine supplementation in solid and liquid culture of M.tb in vitro for advanced immunodiagnostic studies:

Earlier studies using immunogenic excretory-secretory proteins as released in thyroxine supplemented culture medium of M.tb in vitro have shown to be useful for diagnosis of pulmonary and extra-pulmonary tuberculosis ( 1-3 ). The current exploratory study using different fractions of the above mentioned immunogenic proteins showed higher discriminatory efficiency during diagnostic evaluation. Affinity chromatography technique proved to be more effective and less time consuming technique for higher yield of specific antigenic protein fractions compared to conventional protein purification techniques such as Salt precipitation, SDS-PAGE, cation exchange fast protein liquid chromatography(FPLC) etc. Thyroxine supplementation in L-J medium and Sauton's medium for culture of M.tb in vitro, augmented yield of excretory secretory proteins and helped to circumvent the hurdles in progress of research activities as described in the current study (4). However, molecular studies are warranted to explore the effects of thyroxine hormone on the mycobacterial protein synthetic machinery and protein products thereof. Advanced molecular studies may help us to eliminate our worries about genetic manipulation under influence of thyroxine on mycobacterial genome. In such such situation, excretory-secretory protein structures, patterns of protein folding etc., may not be the same and immunogenic properties may change if not evolve to a novel one.

Prof.Dr.J.Pramanik.MD Professor Faculty of Medicine Lincoln University College, Malaysia.

References:

1. Pramanik.J et al.,Increased yield of excretory-secretory antigen with thyroxine supplement in in vitro culture of tubercle bacilli.Indian Journal of Tuberculosis. 1997 Oct; 44(4): 185-90
2. Pramanik.J et al.,Detection of tubercular antibody and antigen in sera of bone and joint tuberculosis: Indian J Clin Biochem. 2000 Aug; 15(1): 22–28.doi: 10.1007/BF02873543,PMCID: PMC3453540. 3.Lodam A.N., Pramanik J.,Reddy M.V.R. et al.,Diagnostic potential of fractionated Mycobacterium tuberculosis H37Ra excretory—secretory (EST-DE1) antigen in pulmonary tuberculosis. Indian. J. Clin. Biochem. 1997;12(1):71–77
3. Nair ER et al.,Isolation of Mycobacterium Tuberculosis 31 kDa antigen protein of diagnostic interest from culture filtrate using anti-ES-31 antibody by affinity chromatography. Indian J Clin Biochem. 2001 Jan;16(1):132-5. doi: 10.1007/BF02867583.

On 2015 Nov 17, Prof.Dr.Jogenananda Pramanik commented:

Thyroxine supplementation in solid and liquid culture of M.tb in vitro for advanced immunodiagnostic studies:

Earlier studies using immunogenic excretory-secretory proteins as released in thyroxine supplemented culture medium of M.tb in vitro have shown to be useful for diagnosis of pulmonary and extra-pulmonary tuberculosis ( 1-3 ). The current exploratory study using different fractions of the above mentioned immunogenic proteins showed higher discriminatory efficiency during diagnostic evaluation. Affinity chromatography technique proved to be more effective and less time consuming technique for higher yield of specific antigenic protein fractions compared to conventional protein purification techniques such as Salt precipitation, SDS-PAGE, cation exchange fast protein liquid chromatography(FPLC) etc. Thyroxine supplementation in L-J medium and Sauton's medium for culture of M.tb in vitro, augmented yield of excretory secretory proteins and helped to circumvent the hurdles in progress of research activities as described in the current study (4). However, molecular studies are warranted to explore the effects of thyroxine hormone on the mycobacterial protein synthetic machinery and protein products thereof. Advanced molecular studies may help us to eliminate our worries about genetic manipulation under influence of thyroxine on mycobacterial genome. In such such situation, excretory-secretory protein structures, patterns of protein folding etc., may not be the same and immunogenic properties may change if not evolve to a novel one.

Prof.Dr.J.Pramanik.MD Professor Faculty of Medicine Lincoln University College, Malaysia.

References:

1. Pramanik.J et al.,Increased yield of excretory-secretory antigen with thyroxine supplement in in vitro culture of tubercle bacilli.Indian Journal of Tuberculosis. 1997 Oct; 44(4): 185-90
2. Pramanik.J et al.,Detection of tubercular antibody and antigen in sera of bone and joint tuberculosis: Indian J Clin Biochem. 2000 Aug; 15(1): 22–28.doi: 10.1007/BF02873543,PMCID: PMC3453540. 3.Lodam A.N., Pramanik J.,Reddy M.V.R. et al.,Diagnostic potential of fractionated Mycobacterium tuberculosis H37Ra excretory—secretory (EST-DE1) antigen in pulmonary tuberculosis. Indian. J. Clin. Biochem. 1997;12(1):71–77
3. Nair ER et al.,Isolation of Mycobacterium Tuberculosis 31 kDa antigen protein of diagnostic interest from culture filtrate using anti-ES-31 antibody by affinity chromatography. Indian J Clin Biochem. 2001 Jan;16(1):132-5. doi: 10.1007/BF02867583.