On 2013 Jul 10, Julia Gilden commented:

This is a nice little paper that might explain the frustrations of researchers having trouble with consistent ectopic expression. The authors speculate in the discussion that reduced RNA Polymerase I transcription at high densities may reflect a developmental transition toward the short stumpy form of the parasite. It is not clear, however, what the authors think this reduced RNA Pol I activity might accomplish, particularly because (as they point out) procyclin transcription is RNA Pol I-dependent, and a switch to the procyclin coat must be induced upon uptake by the fly.

On 2013 Jul 10, Julia Gilden commented:

This is a nice little paper that might explain the frustrations of researchers having trouble with consistent ectopic expression. The authors speculate in the discussion that reduced RNA Polymerase I transcription at high densities may reflect a developmental transition toward the short stumpy form of the parasite. It is not clear, however, what the authors think this reduced RNA Pol I activity might accomplish, particularly because (as they point out) procyclin transcription is RNA Pol I-dependent, and a switch to the procyclin coat must be induced upon uptake by the fly.