On 2014 Feb 20, Koji Ohira commented:

We made the electrophoresis chambers and performed the CLARITY according to the protocol. The chambers were made with the same materials/dimensions as the ones shown in the methods by a manufacturer. However, brains did not become transparent. I think that hydrogel tissue embedding is going well, but electrophoretic tissue clearing (ETC) is not. In our ECT, brain samples were applied with 30V at 37 °C for more than 10 days. Many air bubbles were generated around both electrodes during ETC and seemed to disturb the electricity. In fact, more than 50V cannot apply to the chamber in our system. I heard that some of our colleagues failed to make brain transparent with exactly same problems (i.e. a lot of air bubbles were generated). When you established the method, would you have a similar problem? Any tips? I wonder if I could get your comments and advice about that.

On 2014 Feb 20, Koji Ohira commented:

We made the electrophoresis chambers and performed the CLARITY according to the protocol. The chambers were made with the same materials/dimensions as the ones shown in the methods by a manufacturer. However, brains did not become transparent. I think that hydrogel tissue embedding is going well, but electrophoretic tissue clearing (ETC) is not. In our ECT, brain samples were applied with 30V at 37 °C for more than 10 days. Many air bubbles were generated around both electrodes during ETC and seemed to disturb the electricity. In fact, more than 50V cannot apply to the chamber in our system. I heard that some of our colleagues failed to make brain transparent with exactly same problems (i.e. a lot of air bubbles were generated). When you established the method, would you have a similar problem? Any tips? I wonder if I could get your comments and advice about that.