On 2014 Feb 04, Jean-Jacques Letesson commented:

In the bacteria and plasmid section of this paper, the readers should be aware that it is impossible to grow B. melitensis in the minimal medium stated "B. melitensis 16 M was routinely cultured in rich medium Tryptic Soy Broth (TSB) or in minimal medium GEM7.0 (MgSO4.7H2O 0.2 g/L, Citric acid•H2O 2.0 g/L, K2HPO4 10.0 g/L, NaNH4HPO4.4H2O 3.5 g/L, Glucose 20 g/L, pH 7.0)" All Brucella strictly require biotin, panthotenate, thiamine and nicotinamide as additional factors. In addition 20g/L of glucose is almost ten times as much as normaly needed.

On 2014 Feb 04, Jean-Jacques Letesson commented:

In the bacteria and plasmid section of this paper, the readers should be aware that it is impossible to grow B. melitensis in the minimal medium stated "B. melitensis 16 M was routinely cultured in rich medium Tryptic Soy Broth (TSB) or in minimal medium GEM7.0 (MgSO4.7H2O 0.2 g/L, Citric acid•H2O 2.0 g/L, K2HPO4 10.0 g/L, NaNH4HPO4.4H2O 3.5 g/L, Glucose 20 g/L, pH 7.0)" All Brucella strictly require biotin, panthotenate, thiamine and nicotinamide as additional factors. In addition 20g/L of glucose is almost ten times as much as normaly needed.