On 2015 Dec 21, Amy Baxter commented:

The authors use the same methodology to evaluate their free-flow collection device versus prolonged Buzzy application as they did previously, using their free-flow device versus a standard tourniquet at varying times from 30 seconds to 180s. http://www.ncbi.nlm.nih.gov/pubmed/22135855 They found similar 2.1- 4.2% differences in TP, Alb, k, CA, Na, and Mg when leaving a tourniquet on 2 minutes, as they did with Buzzy in this study which found 1.7-4.2% for TP, Ablumin, and transferrin only. As the article does not reference the previous work by the authors showing the same changes due to tourniquet application, an invited editorial can be found here: http://www.ncbi.nlm.nih.gov/pubmed/24478562 Disclosure: I invented and researched Buzzy. The Buzzy device is used for 120s in this study, which is off-label. We do not recommend any prolonged application of the tourniquet.

On 2015 Dec 21, Amy Baxter commented:

The authors use the same methodology to evaluate their free-flow collection device versus prolonged Buzzy application as they did previously, using their free-flow device versus a standard tourniquet at varying times from 30 seconds to 180s. http://www.ncbi.nlm.nih.gov/pubmed/22135855 They found similar 2.1- 4.2% differences in TP, Alb, k, CA, Na, and Mg when leaving a tourniquet on 2 minutes, as they did with Buzzy in this study which found 1.7-4.2% for TP, Ablumin, and transferrin only. As the article does not reference the previous work by the authors showing the same changes due to tourniquet application, an invited editorial can be found here: http://www.ncbi.nlm.nih.gov/pubmed/24478562 Disclosure: I invented and researched Buzzy. The Buzzy device is used for 120s in this study, which is off-label. We do not recommend any prolonged application of the tourniquet.