On 2014 Mar 10, Madhusudana Girija Sanal commented:

It is a common observation that stressed cells, dying and dead cells start to become fluorescent. So early observation of florescence as a pointer to pluripotency (Oct4-GFP) needs to be substantiated by other experiments. The authors need to provide more evidence (molecular biological and morphological) and details (protocol) regarding STAP during the early hours to days post-stress, because this period seems to be the most critical in reprogramming (compared to characterization of the final product-STAPs). The authors underscore that the production of STAP is not the result of any type of “selection” during culture. However in a subsequent protocol paper (doi:10.1038/protex.2014.008) they see a ‘possibility of negative cell-type-dependent bias’. In the new protocol paper they also didn’t observe any T-Cell receptor rearrangement. So what type of PTPRC (CD45+) cells contributed to STAP? It is also difficult to understand or define the difference between STAP cells and STAP stem cells.

On 2014 Mar 10, Madhusudana Girija Sanal commented:

It is a common observation that stressed cells, dying and dead cells start to become fluorescent. So early observation of florescence as a pointer to pluripotency (Oct4-GFP) needs to be substantiated by other experiments. The authors need to provide more evidence (molecular biological and morphological) and details (protocol) regarding STAP during the early hours to days post-stress, because this period seems to be the most critical in reprogramming (compared to characterization of the final product-STAPs). The authors underscore that the production of STAP is not the result of any type of “selection” during culture. However in a subsequent protocol paper (doi:10.1038/protex.2014.008) they see a ‘possibility of negative cell-type-dependent bias’. In the new protocol paper they also didn’t observe any T-Cell receptor rearrangement. So what type of PTPRC (CD45+) cells contributed to STAP? It is also difficult to understand or define the difference between STAP cells and STAP stem cells.