On 2014 May 14, Jim Woodgett commented:

Two points to consider: 1. Canonical Wnt signaling is not appreciatively activated upon genetic deletion of GSK-3beta due to the redundant action of GSK-3alpha (Axin associates with either isoform). See: PMID: 17543867 2. 6-bromoindirubin 3'-oxime (BIO) is not isoform selective, nor are any other small molecule GSK-3 inhibitors. Hence, it is highly likely that the observed results on expansion of hematopoietic progenitors (also previously observed in animals treated with lithium: PMID: 16341242) are due to inhibition of GSK-3 (rather than GSK-3beta alone).

On 2014 May 14, Jim Woodgett commented:

Two points to consider: 1. Canonical Wnt signaling is not appreciatively activated upon genetic deletion of GSK-3beta due to the redundant action of GSK-3alpha (Axin associates with either isoform). See: PMID: 17543867 2. 6-bromoindirubin 3'-oxime (BIO) is not isoform selective, nor are any other small molecule GSK-3 inhibitors. Hence, it is highly likely that the observed results on expansion of hematopoietic progenitors (also previously observed in animals treated with lithium: PMID: 16341242) are due to inhibition of GSK-3 (rather than GSK-3beta alone).