On 2014 Sep 11, Samir Ounzain commented:

Very nice piece of work from the Visel and Pennacchio laboratories. Enhancer-templated ncRNA transcription is emerging as a common feature associated with active tissue specific enhancers. We have recently using the excellent ChIP-Seq data sets generatated by this laboratory made similiar observations specifically within the heart in various in vivo and in vitro models of cardiac differentiation and pathological remodelling. Interestingly many of these enhancers are not only templating eRNAs, as described in this study, but also PolyA+, unidirectional and multi-exonic lncRNAs. Furthermore loss of function demonstrates these enhancer templated lncRNAs are critical for the regulation of enhancer target coding genes.

I would be fascinated to see how many of the TSTRs identified in this study are producing spliced lncRNAs and furthermore if loss-of-function leads to repressed enhancer activity on target genes.

Great work and I look forward to future studies from this group exploring these possibilities.

For those more interested in heart specific enhancer templated lncRNAs, please refer to our recent publications.

Ounzain, S., Micheletti, R., Beckmann, T., Schroen, B., Alexanian, M., Pezzuto, I., Crippa, S., Nemir, M., Sarre, A., Johnson, R., et al. (2014a). Genome-wide profiling of the cardiac transcriptome after myocardial infarction identifies novel heart-specific long non-coding RNAs. European heart journal.

Ounzain, S., Pezzuto, I., Micheletti, R., Burdet, F., Sheta, R., Nemir, M., Gonzales, C., Sarre, A., Alexanian, M., Blow, M.J., et al. (2014b). Functional importance of cardiac enhancer-associated noncoding RNAs in heart development and disease. Journal of molecular and cellular cardiology 76C, 55-70.

On 2014 Sep 11, Samir Ounzain commented:

Very nice piece of work from the Visel and Pennacchio laboratories. Enhancer-templated ncRNA transcription is emerging as a common feature associated with active tissue specific enhancers. We have recently using the excellent ChIP-Seq data sets generatated by this laboratory made similiar observations specifically within the heart in various in vivo and in vitro models of cardiac differentiation and pathological remodelling. Interestingly many of these enhancers are not only templating eRNAs, as described in this study, but also PolyA+, unidirectional and multi-exonic lncRNAs. Furthermore loss of function demonstrates these enhancer templated lncRNAs are critical for the regulation of enhancer target coding genes.

I would be fascinated to see how many of the TSTRs identified in this study are producing spliced lncRNAs and furthermore if loss-of-function leads to repressed enhancer activity on target genes.

Great work and I look forward to future studies from this group exploring these possibilities.

For those more interested in heart specific enhancer templated lncRNAs, please refer to our recent publications.

Ounzain, S., Micheletti, R., Beckmann, T., Schroen, B., Alexanian, M., Pezzuto, I., Crippa, S., Nemir, M., Sarre, A., Johnson, R., et al. (2014a). Genome-wide profiling of the cardiac transcriptome after myocardial infarction identifies novel heart-specific long non-coding RNAs. European heart journal.

Ounzain, S., Pezzuto, I., Micheletti, R., Burdet, F., Sheta, R., Nemir, M., Gonzales, C., Sarre, A., Alexanian, M., Blow, M.J., et al. (2014b). Functional importance of cardiac enhancer-associated noncoding RNAs in heart development and disease. Journal of molecular and cellular cardiology 76C, 55-70.