On 2014 Dec 10, Alberto Zambrano commented:

Dear Ilya, The Ab used was initially described by Dr. Sonenberg (Mol Cell Biol. 1998 Jan;18(1):334-42, A novel functional human eukaryotic translation initiation factor 4G.) and was kindly donated by Dr. Cesar de Haro (CBM, Madrid, Spain) There you can see that the size of the recombinant form of EIF4G2 (His-tagged) (Figure 3) recognized by the Ab is a little bit lower than 206 KDa. You can also see another prominent band higher than 117KDa, recognized by the Ab. In a another article from the same author (J Neurosci. 2012 Apr 18;32(16):5620-30. doi: 10.1523/JNEUROSCI.0030-12.2012. Regulation of neuronal mRNA translation by CaM-kinase I phosphorylation of eIF4GII) the same Ab was also used but, unfortunately the protein markers were not indicated. In our blots, the Ab reactivity shows either a prominent, discrete band or a doublet at size indicated. We don't observe any other significant reactivity in the full blots. In addition, those bands were sensitive to specific specific siRNA downregulation (figure 5). We don't have a clue about the origin of such differential electroforetic mobility differencies, described in the original paper or in ours (in MEFs, with respect to the predicted size but with the evidences we had, we assumed that protein identity.

On 2014 Dec 01, Ilya M Terenin commented:

Ghm... Guys, why your eIF4G2 is heavier than 150 kDa on blots? The protein is ~100 kDa.

On 2014 Dec 01, Ilya M Terenin commented:

Ghm... Guys, why your eIF4G2 is heavier than 150 kDa on blots? The protein is ~100 kDa.