On 2015 Feb 25, Vincent DiGiacomo commented:

Note that support for the ability of RPSA to homo- and heterodimerize within cells was recently generated using bimolecular fluorescence complementation (BiFC) by Alqahtani F, 2014. It is unclear whether these dimers represent the protein forms referred to as "higher molecular weight (HMW) species" in this review.

For those wishing to examine this evidence, note that HMW RPSA/LAMR species (i.e. 67LR, 67-kDa LAMR, etc.) are generally accepted to be stable under the denaturing and reducing conditions of SDS-PAGE – as noted in the review. Alqahtani F, 2014 helpfully provides an immunoblot detecting the tagged-RPSA constructs within the cells used for the BiFC (supplemental information).

Each individual can evaluate the strength of the new evidence to determine whether it constitutes evidence that the identity of HMW species has been resolved. In my opinion, definitive identification remains unclear.

On 2015 Feb 25, Vincent DiGiacomo commented:

Note that support for the ability of RPSA to homo- and heterodimerize within cells was recently generated using bimolecular fluorescence complementation (BiFC) by Alqahtani F, 2014. It is unclear whether these dimers represent the protein forms referred to as "higher molecular weight (HMW) species" in this review.

For those wishing to examine this evidence, note that HMW RPSA/LAMR species (i.e. 67LR, 67-kDa LAMR, etc.) are generally accepted to be stable under the denaturing and reducing conditions of SDS-PAGE – as noted in the review. Alqahtani F, 2014 helpfully provides an immunoblot detecting the tagged-RPSA constructs within the cells used for the BiFC (supplemental information).

Each individual can evaluate the strength of the new evidence to determine whether it constitutes evidence that the identity of HMW species has been resolved. In my opinion, definitive identification remains unclear.