On 2016 Jun 01, Kenneth Witwer commented:

It is reported that "miR-150 mRNA and protein expression levels" were assayed in this study, but miR-150, a 22-nucleotide RNA, is neither a messenger RNA nor a protein. The reverse transcription and RT-PCR steps described in the methods could not have amplified a microRNA specifically. Furthermore, both Western blots and immunohistochemistry images are shown and said to have been obtained with a "mouse polyclonal anti-miR-150 antibody." The target is repeatedly referred to as a protein, reducing the likelihood that typographical errors or translation issues interfered with reporting. Additional methodologic details would be helpful in resolving these problems, including primer sequences and the actual protein target and source of the antibody.

On 2016 Jun 01, Kenneth Witwer commented:

It is reported that "miR-150 mRNA and protein expression levels" were assayed in this study, but miR-150, a 22-nucleotide RNA, is neither a messenger RNA nor a protein. The reverse transcription and RT-PCR steps described in the methods could not have amplified a microRNA specifically. Furthermore, both Western blots and immunohistochemistry images are shown and said to have been obtained with a "mouse polyclonal anti-miR-150 antibody." The target is repeatedly referred to as a protein, reducing the likelihood that typographical errors or translation issues interfered with reporting. Additional methodologic details would be helpful in resolving these problems, including primer sequences and the actual protein target and source of the antibody.